ID A0A0L9URZ2_PHAAN Unreviewed; 808 AA.
AC A0A0L9URZ2;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=long-chain-alcohol oxidase {ECO:0000256|ARBA:ARBA00013125};
DE EC=1.1.3.20 {ECO:0000256|ARBA:ARBA00013125};
GN ORFNames=LR48_Vigan06g067300 {ECO:0000313|EMBL:KOM45367.1};
OS Phaseolus angularis (Azuki bean) (Vigna angularis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3914 {ECO:0000313|EMBL:KOM45367.1, ECO:0000313|Proteomes:UP000053144};
RN [1] {ECO:0000313|Proteomes:UP000053144}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jingnong 6 {ECO:0000313|Proteomes:UP000053144};
RX PubMed=26460024; DOI=10.1073/pnas.1420949112;
RA Yang K., Tian Z., Chen C., Luo L., Zhao B., Wang Z., Yu L., Li Y., Sun Y.,
RA Li W., Chen Y., Li Y., Zhang Y., Ai D., Zhao J., Shang C., Ma Y., Wu B.,
RA Wang M., Gao L., Sun D., Zhang P., Guo F., Wang W., Li Y., Wang J.,
RA Varshney R.K., Wang J., Ling H.Q., Wan P.;
RT "Genome sequencing of adzuki bean (Vigna angularis) provides insight into
RT high starch and low fat accumulation and domestication.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13213-13218(2015).
CC -!- FUNCTION: Long-chain fatty alcohol oxidase involved in the omega-
CC oxidation pathway of lipid degradation.
CC {ECO:0000256|ARBA:ARBA00003842}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain primary fatty alcohol + O2 = a long-chain fatty
CC aldehyde + H2O2; Xref=Rhea:RHEA:22756, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17176, ChEBI:CHEBI:77396; EC=1.1.3.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000920};
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790}.
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DR EMBL; CM003376; KOM45367.1; -; Genomic_DNA.
DR RefSeq; XP_017426124.1; XM_017570635.1.
DR AlphaFoldDB; A0A0L9URZ2; -.
DR STRING; 3914.A0A0L9URZ2; -.
DR EnsemblPlants; KOM45367; KOM45367; LR48_Vigan06g067300.
DR GeneID; 108334714; -.
DR Gramene; KOM45367; KOM45367; LR48_Vigan06g067300.
DR KEGG; var:108334714; -.
DR OMA; ASEVWPE; -.
DR OrthoDB; 601859at2759; -.
DR Proteomes; UP000053144; Chromosome 6.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046577; F:long-chain-alcohol oxidase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR InterPro; IPR012400; Long_Oxdase.
DR PANTHER; PTHR46056; LONG-CHAIN-ALCOHOL OXIDASE; 1.
DR PANTHER; PTHR46056:SF4; LONG-CHAIN-ALCOHOL OXIDASE; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF028937; Lg_Ch_AO; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|PIRSR:PIRSR028937-2};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR028937-2};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000053144};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 344..564
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00732"
FT DOMAIN 664..793
FT /note="Glucose-methanol-choline oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05199"
FT ACT_SITE 739
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR028937-1"
FT BINDING 297..312
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR028937-2"
SQ SEQUENCE 808 AA; 88434 MW; 1F4BF333E5309B74 CRC64;
MEDRTIGRSG SSRKGSFRFG IGVDGKKDHT VVELGSIDTH RLLLSGGERQ KKQQPLTNSL
SSRQMKSLSA LCDSILPSVD HSVHTSDESV NKFYRISASM AGTPELLGGV MCEKLKHPLT
GLLKLALWGL STWFGTLILC GMGCVSTKFP FIHSFPELSL QKRQDIMRSW SLSYIRLLRL
LFRTIKLLTL LVFFTQTDES EENLSWKAIG YCGPDPKFKA QLKNHFLDGK SKEAEDDKED
EDAEEVIGPL YKGLVHLNNP RDIAADALRR LGFSVSVVRR KHKAAANLSS PSLVIQCDAV
VVGSGSGGGV IAGVLAKAGY KVLVLEKGGY SARNNLSLLE GQSMDQMYLN AGLVASDDMG
MFILSGSTVG GGSAVNWSAS IKTPQHVCKE WCEKHGLELF ESELYREALD AVCEKMGVQS
EIEDEGFNNA VLRKGCQEMG YPVNTIPRNS SSDHYCGWCC MGCKDGKKKG TLETWLVDLV
KSGNGAILPG CEALKVLHKN MEGRDRKVAG GVAFEFEYKG IKDICVVESK VTIVACGALC
TPALLKRSGL RNQNIGRNLH LHPVTMAWGY FPDEPASEVW PEANKKSYEG GIMTAMSSVV
AEFEQSGYGA VIQTPSLHPG MFSIVTPWTS GVDIRDRMRK FSRTAHVFAL ARDKGSGTVE
SPHRISYKLA GVDEKNLQKG IDKVLRILAA AGAEEIGTHH NKGRTLNVKQ VSYHEFEKFV
KEESSRPLTD LSTPLCSAHQ MGSCRMGSNP KQSVVNQTGE TWEMEGLYVA DTSVFPTALG
VNPMVTVQAI AYCVAQSVLE VLRRKRSK
//
