ID A0A0L9VJS0_PHAAN Unreviewed; 538 AA.
AC A0A0L9VJS0;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN ORFNames=LR48_Vigan10g105400 {ECO:0000313|EMBL:KOM55162.1};
OS Phaseolus angularis (Azuki bean) (Vigna angularis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3914 {ECO:0000313|EMBL:KOM55162.1, ECO:0000313|Proteomes:UP000053144};
RN [1] {ECO:0000313|Proteomes:UP000053144}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jingnong 6 {ECO:0000313|Proteomes:UP000053144};
RX PubMed=26460024; DOI=10.1073/pnas.1420949112;
RA Yang K., Tian Z., Chen C., Luo L., Zhao B., Wang Z., Yu L., Li Y., Sun Y.,
RA Li W., Chen Y., Li Y., Zhang Y., Ai D., Zhao J., Shang C., Ma Y., Wu B.,
RA Wang M., Gao L., Sun D., Zhang P., Guo F., Wang W., Li Y., Wang J.,
RA Varshney R.K., Wang J., Ling H.Q., Wan P.;
RT "Genome sequencing of adzuki bean (Vigna angularis) provides insight into
RT high starch and low fat accumulation and domestication.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13213-13218(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; CM003380; KOM55162.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0L9VJS0; -.
DR STRING; 3914.A0A0L9VJS0; -.
DR EnsemblPlants; KOM55162; KOM55162; LR48_Vigan10g105400.
DR Gramene; KOM55162; KOM55162; LR48_Vigan10g105400.
DR OMA; LFWQYGQ; -.
DR Proteomes; UP000053144; Chromosome 10.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451:SF49; MANNAN ENDO-1,4-BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000053144};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..25
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 75..376
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 538 AA; 61407 MW; 164ECF3D4645B4E3 CRC64;
MVFGNSLFYP ILGFASFVVF VYMSFGGIRF SFLEEGPELS FVERNGTQFV LDGKAFYVNG
WNSYWLMVQS VDVYSRSKVR EMMKTGAKMG LTVCRTWAFN DGDYNALQTS PGHFDEQAFQ
ALDYVIAEAR QHGIRLLLSL VNNLQAYGGK SQYVKWAWQE GVGLSSSNDS FFFDPSIRTY
FKNYIKTVLT RKNSITGIEY RNDPTIFGWE LINEPRCMSD PSGDTLQGWI DEMSSFVKLI
DTNHLLTVGL EGFYGPNDPK SSTVNPELWA SRLGSDFIRN SKISNIDFAS VHIYPDHWFH
EQIFEDQLKF VSKWMLSHIE DGDKVLKKPV LFSEFGLSEE NQNFSVSDRE KMHRAVLDII
YKSAKRNRSG AGALVWQFLV SGMKEFSDEY GMVPWESSST ASVFVEQSCR LANAKGWTQL
DLVPDECVEG PQHRSFLVAL EQLGLYASLL AEAKETNSEL KLKLQETISI HLSYEAKHAE
SAQFLVKARQ IMTDTVDAMR SLKQQNNDVT EENRLLKARL IELSFRKPVG RLLSRRML
//
