ID   A0A0M0J6T9_9EUKA        Unreviewed;       784 AA.
AC   A0A0M0J6T9;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   22-FEB-2023, entry version 19.
DE   SubName: Full=Glucosylceramidase {ECO:0000313|EMBL:KOO22316.1};
GN   ORFNames=Ctob_001363 {ECO:0000313|EMBL:KOO22316.1};
OS   Chrysochromulina tobinii.
OC   Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales;
OC   Chrysochromulinaceae; Chrysochromulina.
OX   NCBI_TaxID=1460289 {ECO:0000313|EMBL:KOO22316.1, ECO:0000313|Proteomes:UP000037460};
RN   [1] {ECO:0000313|Proteomes:UP000037460}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CCMP291 {ECO:0000313|Proteomes:UP000037460};
RX   PubMed=26397803; DOI=10.1371/journal.pgen.1005469;
RA   Hovde B.T., Deodato C.R., Hunsperger H.M., Ryken S.A., Yost W., Jha R.K.,
RA   Patterson J., Monnat R.J. Jr., Barlow S.B., Starkenburg S.R.,
RA   Cattolico R.A.;
RT   "Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin:
RT   Metabolic Tools for Enhanced Algal Fitness in the Prominent Order
RT   Prymnesiales (Haptophyceae).";
RL   PLoS Genet. 11:e1005469-e1005469(2015).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC       {ECO:0000256|ARBA:ARBA00005382}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KOO22316.1}.
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DR   EMBL; JWZX01003288; KOO22316.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0M0J6T9; -.
DR   OrthoDB; 5487508at2759; -.
DR   Proteomes; UP000037460; Unassembled WGS sequence.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR   GO; GO:0006665; P:sphingolipid metabolic process; IEA:InterPro.
DR   CDD; cd00161; RICIN; 1.
DR   Gene3D; 2.80.10.50; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR033452; GH30_C.
DR   InterPro; IPR001139; Glyco_hydro_30.
DR   InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   InterPro; IPR000772; Ricin_B_lectin.
DR   PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR   PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF02055; Glyco_hydro_30; 1.
DR   Pfam; PF17189; Glyco_hydro_30C; 1.
DR   Pfam; PF00652; Ricin_B_lectin; 1.
DR   PRINTS; PR00843; GLHYDRLASE30.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR   PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000037460};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          27..399
FT                   /note="Glycosyl hydrolase family 30 TIM-barrel"
FT                   /evidence="ECO:0000259|Pfam:PF02055"
FT   DOMAIN          425..525
FT                   /note="Ricin B lectin"
FT                   /evidence="ECO:0000259|Pfam:PF00652"
FT   DOMAIN          593..642
FT                   /note="Glycosyl hydrolase family 30 beta sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF17189"
SQ   SEQUENCE   784 AA;  83915 MW;  4216C57D1ACDDF4B CRC64;
     MPPLEMVPMG SAPKSLAVDT SRRHQEIIGF GGAFTEAAAL NWKLLSEDDQ KKVISLYFDP
     PEKGGHGYTL GRVPINSCDF SPESYSFDDV AGDVELEHFD TSVRHDVESG MIPMILAAQE
     KVVARGEKLN VYASPWSPPA WMKLPAANQS GVVVQSMLVS AKPNGLDPQH QRPWAKYFSK
     FISAYRSHGI DLWGVTVQNE PEAAVGWEAM LWTPEYMASF VRDHLGPVLA AEQPNVKILG
     FDHNKDHVAD WAKALYEDPE AAKFFAGMGV HWYGGLNTHA LDYTHHLQPN KMILGTEACN
     CGGVTYRNIS YKDGAIISSP IAAWWSRAES LALDILEDLR FWAVGWTDWN LVLSIKGGPN
     HLKNLCDANI IVDPINNLSQ GTLIMQASYY YMGHFSRYVP PGSRRVSLNN SVELNAPPLS
     ADDVKNGQAL LFAPCDGDDV QKWTLDDGGS LFVHGTAEAL LSEGYKHGGE CVDVALDSPI
     DGKTQTWACF HVGNQIWSVV DVPGGSQIKN PYLNKCMTAV QTNGAAVGLD PGVAIVAAHV
     KPCLDPGDPS QTFRLANYDE QGFPGNFPVR TVPAAPGGGE LCLQPMIIQV PHFNAVAFEQ
     PSGEVSLITM NLGDSDVNFT IVDQNAQTGV SHTIGSHAIH TYRWKPTARA ADQPDNEPGD
     VAATLITMPV EAQAGSKAVV EQVAVPALVT IEAVRAAGDA QPAAAAAAAA AAAAAAAAAA
     LVPPQPAGET GTTLSSAVLL MMAAGAIATM AVVRHGAAGS RRYERGVDGE LVDDEYTEWT
     PQPA
//