ID A0A0M0JAB2_9EUKA Unreviewed; 847 AA.
AC A0A0M0JAB2;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Xaa-Pro dipeptidase {ECO:0000256|ARBA:ARBA00044141};
DE EC=3.4.13.9 {ECO:0000256|ARBA:ARBA00044051};
DE AltName: Full=Imidodipeptidase {ECO:0000256|ARBA:ARBA00044351};
DE AltName: Full=Peptidase D {ECO:0000256|ARBA:ARBA00044252};
DE AltName: Full=Proline dipeptidase {ECO:0000256|ARBA:ARBA00044284};
GN ORFNames=Ctob_002008 {ECO:0000313|EMBL:KOO23491.1};
OS Chrysochromulina tobinii.
OC Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales;
OC Chrysochromulinaceae; Chrysochromulina.
OX NCBI_TaxID=1460289 {ECO:0000313|EMBL:KOO23491.1, ECO:0000313|Proteomes:UP000037460};
RN [1] {ECO:0000313|Proteomes:UP000037460}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CCMP291 {ECO:0000313|Proteomes:UP000037460};
RX PubMed=26397803; DOI=10.1371/journal.pgen.1005469;
RA Hovde B.T., Deodato C.R., Hunsperger H.M., Ryken S.A., Yost W., Jha R.K.,
RA Patterson J., Monnat R.J. Jr., Barlow S.B., Starkenburg S.R.,
RA Cattolico R.A.;
RT "Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin:
RT Metabolic Tools for Enhanced Algal Fitness in the Prominent Order
RT Prymnesiales (Haptophyceae).";
RL PLoS Genet. 11:e1005469-e1005469(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + Xaa-L-Pro dipeptide = an L-alpha-amino acid + L-proline;
CC Xref=Rhea:RHEA:76407, ChEBI:CHEBI:15377, ChEBI:CHEBI:59869,
CC ChEBI:CHEBI:60039, ChEBI:CHEBI:195196; EC=3.4.13.9;
CC Evidence={ECO:0000256|ARBA:ARBA00043793};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the peptidase M24B family. Eukaryotic-type
CC prolidase subfamily. {ECO:0000256|ARBA:ARBA00043990}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOO23491.1}.
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DR EMBL; JWZX01003186; KOO23491.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M0JAB2; -.
DR OrthoDB; 1377484at2759; -.
DR Proteomes; UP000037460; Unassembled WGS sequence.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:InterPro.
DR CDD; cd01087; Prolidase; 1.
DR Gene3D; 3.90.230.10; Creatinase/methionine aminopeptidase superfamily; 1.
DR Gene3D; 3.40.350.10; Creatinase/prolidase N-terminal domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR007865; Aminopep_P_N.
DR InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000994; Pept_M24.
DR PANTHER; PTHR48392; -; 1.
DR PANTHER; PTHR48392:SF2; PEPTIDASE D; 1.
DR Pfam; PF05195; AMP_N; 1.
DR Pfam; PF01494; FAD_binding_3; 1.
DR Pfam; PF00557; Peptidase_M24; 1.
DR SMART; SM01011; AMP_N; 1.
DR SUPFAM; SSF55920; Creatinase/aminopeptidase; 1.
DR SUPFAM; SSF53092; Creatinase/prolidase N-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000037460}.
FT DOMAIN 1..126
FT /note="Aminopeptidase P N-terminal"
FT /evidence="ECO:0000259|SMART:SM01011"
SQ SEQUENCE 847 AA; 93187 MW; 52B2F9894260AF72 CRC64;
MGAAGHADGV ILLRGGAEVY RYDSDHEPVF RQESYFAHLF GVKEPDCWAM IELPSGRSTL
FIPKLAPEYA IWMGEIKAPS WFEQHYGVDA VLFVNELQAA VGGAVGSATP SQTPIYILSG
TNSDSGLDLA TVLPITEGVP AGSIVDSRSL YTVAAECRVR KSTEEVEVMR YAAWVSSCAH
SSVMREARAG MLEYQLEALF LFHCAFHGGC RHQAYTCICA CGPDGAVLHY GHAGAPNERQ
LLETDMALLD MGCEYQFYAS DITCSFPLSD EFSADQRVVY EAVLDSQRQI LNAMRPGVPW
SDMHRLMWRV TLTHLSKAGL VRGDLEAMLE ANVGYIFTPH GLGHLIGIDT HDVGGYLPHT
PKRSDVNGLK RLRTARVLEA GMVLTVEPGC YFIDALLEPA LAEGSAYRQY LVPEVEVYDS
LSSVQQDDLE SYAIGINPRG MATLKRVDPN LEKEIDVSTG MIDAWMIMAP PMKKIARMES
GTVMGTTRGS VVQGLYKIAI ATPGIEIKLG HKLVSADVEN STLTFFADGT QHVVDYSRSR
LIDATGCWSK LRTAVAAADP TFKVETWPWE IHFRNLFTSD PPSGGHTLKP KDHYIFTTAG
IYCAVLTGQR WAFSLSVNKT LQADGDWMLA DAPTPELVAK LKAHMREHCP PAVSMIEEEE
YARFFTRRSF TGQVVRVSRL NYGERVCFLG DAAHSTIPST GEGMNAALED VRVLIDALGS
SPGGASGPWF ATFNQRRLAD VQAISSYAAY LLEGMKADAA ERNRRTATMV LSLIGQSMGL
LGPTWNDKTF GKLAVKMAPY SEVYEEWQSQ MKKVQPWGNR LIWYFAKDGG RKQVLAQRRA
AKLADAT
//
