ID A0A0M0JUS9_9EUKA Unreviewed; 575 AA.
AC A0A0M0JUS9;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=asparagine--tRNA ligase {ECO:0000256|ARBA:ARBA00012816};
DE EC=6.1.1.22 {ECO:0000256|ARBA:ARBA00012816};
GN ORFNames=Ctob_014277 {ECO:0000313|EMBL:KOO30082.1};
OS Chrysochromulina tobinii.
OC Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales;
OC Chrysochromulinaceae; Chrysochromulina.
OX NCBI_TaxID=1460289 {ECO:0000313|EMBL:KOO30082.1, ECO:0000313|Proteomes:UP000037460};
RN [1] {ECO:0000313|Proteomes:UP000037460}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CCMP291 {ECO:0000313|Proteomes:UP000037460};
RX PubMed=26397803; DOI=10.1371/journal.pgen.1005469;
RA Hovde B.T., Deodato C.R., Hunsperger H.M., Ryken S.A., Yost W., Jha R.K.,
RA Patterson J., Monnat R.J. Jr., Barlow S.B., Starkenburg S.R.,
RA Cattolico R.A.;
RT "Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin:
RT Metabolic Tools for Enhanced Algal Fitness in the Prominent Order
RT Prymnesiales (Haptophyceae).";
RL PLoS Genet. 11:e1005469-e1005469(2015).
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOO30082.1}.
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DR EMBL; JWZX01002297; KOO30082.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M0JUS9; -.
DR OrthoDB; 2786597at2759; -.
DR Proteomes; UP000037460; Unassembled WGS sequence.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd04318; EcAsnRS_like_N; 1.
DR CDD; cd01200; WHEPGMRS_RNA; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 1.10.287.10; S15/NS1, RNA-binding; 1.
DR HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR009068; uS15_NS1_RNA-bd_sf.
DR InterPro; IPR000738; WHEP-TRS_dom.
DR NCBIfam; TIGR00457; asnS; 1.
DR PANTHER; PTHR22594:SF34; ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED; 1.
DR PANTHER; PTHR22594; ASPARTYL/LYSYL-TRNA SYNTHETASE; 1.
DR Pfam; PF00152; tRNA-synt_2; 2.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR Pfam; PF00458; WHEP-TRS; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SMART; SM00991; WHEP-TRS; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF47060; S15/NS1 RNA-binding domain; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR PROSITE; PS51185; WHEP_TRS_2; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:KOO30082.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000037460};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..575
FT /note="asparagine--tRNA ligase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5005602104"
FT DOMAIN 249..309
FT /note="WHEP-TRS"
FT /evidence="ECO:0000259|PROSITE:PS51185"
FT DOMAIN 346..567
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
SQ SEQUENCE 575 AA; 61853 MW; C0A855C3556A91E5 CRC64;
MLRLLACSTM LMGVSGVRSA TRIWQQRGSS LAVSPDFAGL VGRQRSRMLC ASSIETPAPV
AAAMAATPAL KRRKVGSLLA EGESAIGTQV LLKGWVRTVR NQKTFSFVEL NDGSSLAGLQ
VVAPSEIGTY ATVEALTTGA AVSVVGKVVA SQGKGQALEV QAQSVELVGD CPADTYPLQK
KRHSIEFLRS IAHLRPRSNL FGAVSRVRSA LAQATHAFFA SEGFKYVQTP LITASDCEGA
GEMFRVTTLP LTLEGEIASQ GVKVRDLKAA AKEGNAEKSD VEAAVAKLLE LKAKAAAPPP
AAAAAPAPPN MGSSDPYAAD FFGRAAYLTV SGQLSAETYA CALGDVYTFG PTFRAENSNT
ARHLAEFWMI EPEMAFADLA ADMNNAEAFV KYVVRHVLEH CAEDMAYFSS LDKELAGRLE
RLVRDDFARV SYTRAIDLLQ AEIAKDPKQW ENPEVVFGTD LATEHERWLA EKHFGTATFV
YNYPKAIKAF YMRDNDGCEP GKETVAAMDL LVPGIGELIG GSQREERLDK LLSKMAGVPH
AGYGLGFERL VCYVTGVTNI RDAIAFPRFP GSCEF
//
