ID A0A0M2H1H1_9MICO Unreviewed; 353 AA.
AC A0A0M2H1H1;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=3-hydroxyisobutyryl-CoA hydrolase {ECO:0000256|ARBA:ARBA00011915};
DE EC=3.1.2.4 {ECO:0000256|ARBA:ARBA00011915};
GN Name=echA8_1 {ECO:0000313|EMBL:KJL40099.1};
GN ORFNames=RS81_01688 {ECO:0000313|EMBL:KJL40099.1};
OS Microbacterium ketosireducens.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=92835 {ECO:0000313|EMBL:KJL40099.1, ECO:0000313|Proteomes:UP000033956};
RN [1] {ECO:0000313|EMBL:KJL40099.1, ECO:0000313|Proteomes:UP000033956}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12510 {ECO:0000313|EMBL:KJL40099.1,
RC ECO:0000313|Proteomes:UP000033956};
RA Corretto E.;
RT "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT metal contaminated environments.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-
CC methylpropanoate + CoA + H(+); Xref=Rhea:RHEA:20888,
CC ChEBI:CHEBI:11805, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57340; EC=3.1.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001709};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJL40099.1}.
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DR EMBL; JYIZ01000047; KJL40099.1; -; Genomic_DNA.
DR RefSeq; WP_045275631.1; NZ_JYIZ01000047.1.
DR AlphaFoldDB; A0A0M2H1H1; -.
DR STRING; 92835.RS81_01688; -.
DR PATRIC; fig|92835.4.peg.1708; -.
DR OrthoDB; 9790967at2; -.
DR Proteomes; UP000033956; Unassembled WGS sequence.
DR GO; GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:InterPro.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR CDD; cd06558; crotonase-like; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR045004; ECH_dom.
DR InterPro; IPR032259; HIBYL-CoA-H.
DR PANTHER; PTHR43176:SF3; 3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43176; 3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED; 1.
DR Pfam; PF16113; ECH_2; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lyase {ECO:0000313|EMBL:KJL40099.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000033956}.
FT DOMAIN 18..343
FT /note="Enoyl-CoA hydratase/isomerase"
FT /evidence="ECO:0000259|Pfam:PF16113"
SQ SEQUENCE 353 AA; 37500 MW; 4CC1482F5839AA0D CRC64;
MTDAPEPTVL VRSAGGLGRL TLNRPRAINA LDLDMVRQLS AALDAWETDT DVELVVLDGA
GERGLCAGGD VRVLAERVVG GRFDEAALFF REEYALNARI AEYPKPIVAL ADGITMGGGI
GLAGHAAIRV VTERSQLAMP ETRIGFTPDV GGTWLLAHAP GHLGEYLALT GAVMDAADAV
YAGLADHLVP VDHLDGLREA FETRADPSSP TELVLLFDET PAPSALERSR AWIDDAFAAD
TVAEIVERLR ARPEPEASAT ADLLGELSPT GLAVTLAAVR RARGLAGLRD ALAQEYGLVM
WFAQTQPDLA EGIRAQVVDK DRSPRWQPAA LADLDPGIAD QAFAHVPAEP LWS
//
