ID A0A0M2H7P2_9MICO Unreviewed; 402 AA.
AC A0A0M2H7P2;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Putative acyltransferase {ECO:0000313|EMBL:KJL40130.1};
DE EC=2.3.1.- {ECO:0000313|EMBL:KJL40130.1};
GN Name=fadA_2 {ECO:0000313|EMBL:KJL40130.1};
GN ORFNames=RS81_01720 {ECO:0000313|EMBL:KJL40130.1};
OS Microbacterium ketosireducens.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=92835 {ECO:0000313|EMBL:KJL40130.1, ECO:0000313|Proteomes:UP000033956};
RN [1] {ECO:0000313|EMBL:KJL40130.1, ECO:0000313|Proteomes:UP000033956}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12510 {ECO:0000313|EMBL:KJL40130.1,
RC ECO:0000313|Proteomes:UP000033956};
RA Corretto E.;
RT "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT metal contaminated environments.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJL40130.1}.
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DR EMBL; JYIZ01000047; KJL40130.1; -; Genomic_DNA.
DR RefSeq; WP_045275657.1; NZ_JYIZ01000047.1.
DR AlphaFoldDB; A0A0M2H7P2; -.
DR STRING; 92835.RS81_01720; -.
DR PATRIC; fig|92835.4.peg.1739; -.
DR OrthoDB; 3204099at2; -.
DR Proteomes; UP000033956; Unassembled WGS sequence.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR43365; BLR7806 PROTEIN; 1.
DR PANTHER; PTHR43365:SF1; STEROID 3-KETOACYL-COA THIOLASE FADA6; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:KJL40130.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000033956};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:KJL40130.1}.
FT DOMAIN 5..228
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 280..401
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 91
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 358
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 388
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 402 AA; 41899 MW; D0C3155417CA01F7 CRC64;
MPEAYIYDAV RTPRGRNKGG SLHSVKPVDL VVGLIDALRE RNPSLRADLI DDIVLGVVSP
VGEQGGDIAR TAALVAGLPE TVAGVQVNRF CASGLEAVNL AAQKVASGYE DLVLAGGVES
MSRVPIGSDG GAYAQDPTTG YDHYFVPQGI GADLIATIEG FSREDVDAFA ARSQARADTA
WREGRFAASV VPVTDINGVT LLAEDEHRRP GSTVESLGQL PASFAALGEQ AGFDAVALQK
YHWVEKIDHV HGPGNSSGIV DGAALVVIGT REIGERLGLT PRARIVSTAV TGSDPTIMLT
GPTPATQKAL EKAGLDASDI DLFELNEAFA AVVMKWQKDL GIPDDKVNVN GGAIALGHPL
GATGAMILGT LLDELERQDL RRGLATLCVG AGMGIATVIE RV
//
