ID   A0A0M2HGC9_9MICO        Unreviewed;       698 AA.
AC   A0A0M2HGC9;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   SubName: Full=Glycogen debranching enzyme {ECO:0000313|EMBL:KJL43354.1};
DE            EC=3.2.1.- {ECO:0000313|EMBL:KJL43354.1};
GN   Name=glgX_1 {ECO:0000313|EMBL:KJL43354.1};
GN   ORFNames=RS82_01406 {ECO:0000313|EMBL:KJL43354.1};
OS   Microbacterium trichothecenolyticum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=69370 {ECO:0000313|EMBL:KJL43354.1, ECO:0000313|Proteomes:UP000034098};
RN   [1] {ECO:0000313|EMBL:KJL43354.1, ECO:0000313|Proteomes:UP000034098}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 8608 {ECO:0000313|EMBL:KJL43354.1,
RC   ECO:0000313|Proteomes:UP000034098};
RA   Corretto E.;
RT   "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT   metal contaminated environments.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJL43354.1}.
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DR   EMBL; JYJA01000031; KJL43354.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0M2HGC9; -.
DR   PATRIC; fig|69370.6.peg.1442; -.
DR   Proteomes; UP000034098; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000313|EMBL:KJL43354.1};
KW   Hydrolase {ECO:0000313|EMBL:KJL43354.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000034098}.
FT   DOMAIN          127..574
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          475..497
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        478..492
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   698 AA;  77192 MW;  7B813136C0FBD1A4 CRC64;
     MVVRMISPVS VISPPDADLL DPRLDGLGVV LLDGHARLRV WSDAADAIDL VVFDDTDLDW
     ITATEPLVAI GGGLWEITTP LLRPGTRYAI RVDGPQAPGN TFNRGTLLLE PYSRGLVRTG
     YDGWRSEVVD GSFDWGGVAK PAIPMDRTVL YEGHLKGLSK RHPDVPGALR GTYAGLAHPA
     MVQHLLDLGV TSIELLPIHA FTTEPRLLQH GLTNYWGYNS LNFFTPHAAY ATEDACRQGP
     EAILREFKGM VKLLHEAGLE VILDVVYNHT AEEGIGGPRS SLRGIDNRAY YRQQEDGAYI
     DVTGCGNSVN TSTDAAARLV LDSLRYWAND VQIDGFRFDL AATLGRDAGH VFTPQHPLLR
     AILDDPALAG VKMIAEPWDV GMGGWQTGNF GDGWSEWNDR YRDRVRNFWL SDVDYARRAS
     TSPVGIGGFA NRLAGSANTF ADVRGPLAGI NFVTAHDGFT LRDLVSYDVK HNLGNGEQNR
     DGADTNRSFN HGAEGPTDDE RILATRRKAM RNLLGTLLLS AGVPMLTAGD EFARTQRGNN
     NAYCHDSVLT WLSWEHAPWQ QDLYAHVRHL LRMRRENPAL RPSRFARLGE RTPSASVMEW
     YDERGETMSI ERWTDPSHRT LQYVAASTPE FEGFNRVLLM IHGTERAIDV TLPDVEDVTR
     YVSLWSSADE TPSESGETYA PGDTVSLAGT SMRLFRAE
//