ID A0A0M2X8U9_9CORY Unreviewed; 308 AA.
AC A0A0M2X8U9;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=D4R08_01380 {ECO:0000313|EMBL:AYJ32165.1}, HF852_06530
GN {ECO:0000313|EMBL:NMF09255.1};
OS Corynebacterium xerosis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=1725 {ECO:0000313|EMBL:NMF09255.1, ECO:0000313|Proteomes:UP000589552};
RN [1] {ECO:0000313|EMBL:AYJ32165.1, ECO:0000313|Proteomes:UP000269054}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS 1 {ECO:0000313|EMBL:AYJ32165.1,
RC ECO:0000313|Proteomes:UP000269054};
RA Wen F.;
RT "Isolation and Identification of Corynebacterium Xerosis Isolated from Yak
RT Liver.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:NMF09255.1, ECO:0000313|Proteomes:UP000589552}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BL-383-APC-2I {ECO:0000313|EMBL:NMF09255.1,
RC ECO:0000313|Proteomes:UP000589552};
RA Hitch T.C.A., Wylensek D., Clavel T.;
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; CP032788; AYJ32165.1; -; Genomic_DNA.
DR EMBL; JABAGA010000003; NMF09255.1; -; Genomic_DNA.
DR RefSeq; WP_046651556.1; NZ_LAYS01000146.1.
DR AlphaFoldDB; A0A0M2X8U9; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000269054; Chromosome.
DR Proteomes; UP000589552; Unassembled WGS sequence.
DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 90..244
FT /note="Pseudouridine synthase RsuA/RluA-like"
FT /evidence="ECO:0000259|Pfam:PF00849"
FT ACT_SITE 140
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 308 AA; 33220 MW; A0CB5284C634C47E CRC64;
MRESRQMPVP PGLAGMRVDA GLAKLLGLSR TAAAELAEAG DVQVDGVDVG KSDRLREDAW
LSVLLPEVQA PLMPKEELVE GMDVLYHDND LVAVHKPVGV AAHPSVGWTG PTVIGGLAAA
GFRISTSGPP ERQGIVQRLD VGTSGVMVVA CSERGYSVLK RAFKNREVSK TYHALVQGHP
DPSSGTIDAA IGRHPSAGWR FCVRRDGKPA VTHYKTLEAF APATLLEVGL ETGRTHQIRV
HFSALNHPCC GDPMYGSDEA LAEKLGLTRQ WLHAVSVGFR HPADGRWMEI ECPYPDDLQH
ALDVLRAQ
//
