ID A0A0M8SKP2_9ACTN Unreviewed; 952 AA.
AC A0A0M8SKP2;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Penicillin amidase {ECO:0000313|EMBL:KOU34593.1};
GN ORFNames=ADK51_06725 {ECO:0000313|EMBL:KOU34593.1};
OS Streptomyces sp. WM6368.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1415554 {ECO:0000313|EMBL:KOU34593.1, ECO:0000313|Proteomes:UP000037718};
RN [1] {ECO:0000313|EMBL:KOU34593.1, ECO:0000313|Proteomes:UP000037718}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WM6368 {ECO:0000313|EMBL:KOU34593.1,
RC ECO:0000313|Proteomes:UP000037718};
RA Noorani M.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOU34593.1}.
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DR EMBL; LGDA01000036; KOU34593.1; -; Genomic_DNA.
DR RefSeq; WP_053701846.1; NZ_LGDA01000036.1.
DR AlphaFoldDB; A0A0M8SKP2; -.
DR PATRIC; fig|1415554.3.peg.1410; -.
DR Proteomes; UP000037718; Unassembled WGS sequence.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 2.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2}.
FT REGION 246..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 549..571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..273
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 332
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 210
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 410
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 413
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 952 AA; 102836 MW; F3AE0BDFA6F97CB5 CRC64;
MPANETAPPV KKKGRRARLI VLVLVLALVA GLGYGAYWSV DGVRASFPQT TGQLKVPGLT
GTVEVKRDAR GIPQLYADSD DDLFRAQGFV HAQDRFWEMD VRRHMTSGRL SEMFGSGQVE
TDAFLRTLGW RQVAQAEFDT KLSPETKKYL QAYADGVNAY LKGKSGKDLS VEHAALKLSD
DYRPEQWSPV DSVAWLKAMA WDLRGNMQDE IDRALMATKL SQAQIDELYP PYPFDRNKPI
VEGGKVDGGK YTPQGGAAGG GNGTGTGTGS GSGNAVGTQT VTPTGTGAGT GLADNATAQG
ATVGLRTQLT ALSDTLDKIP AILGPNGSGI GSNSWVISGK YTTTGKPLLA NDPHLSPQLP
SVWYQMGLHC RTASAQCQYD VAGYTFSGMP GVVIGHNADI AWGMTNLGAD VTDLYLEQVK
PEGYVYDNRV LPFTTREEVI KVAGGASKKI TVRTTNNGPL VSDRSEELGT VGTRAPVASA
APDRGDGYAV ALRWTALDPG RTMDAVFKLN RAKDFPSFRA AAAEFDVPSQ NLIYADNKGA
SGNIGYQAPG RIPVRGQGDG RMPTPGWDSK YAWKGGKDGN TGYIPQNEMP WDSNPSRGYI
VTANQAVVET GTGAGKYPYL LTTDWGYGAR SQRINDLIEA KIKDGGRIST DDMRTMQMDN
SSEIAALLTP MLSKIEISDP GVRAAQKLLD GWNYTQESDS AAAAYFNAVW RNILKLSFGD
KMPKELRLEG SCMNVLGNGT GPADDLAKTV RECGTRDADS AQPDGGDRWF EVVRRLVKDE
KSPWWSAPAK GLNKPATTTR DQLFARAMAD ARWELTAKLG KDQSTWSWGR LHQLTLKNQT
IGTEGPGFMQ WLLNRGPWNL GGGEATVNAT GWNASSGYGV TWVPSMRMVV NLNELDKSRW
INLTGASGHA YNAHYTDQTT MWAKGELLEW PFGKDAVEKA TVDTLTLTPE GS
//
