ID   A0A0M8Y105_9PSEU        Unreviewed;       636 AA.
AC   A0A0M8Y105;
DT   09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT   09-DEC-2015, sequence version 1.
DT   24-JAN-2024, entry version 24.
DE   RecName: Full=Arabinogalactan endo-beta-1,4-galactanase {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192};
DE            EC=3.2.1.89 {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192};
GN   ORFNames=ADK67_41890 {ECO:0000313|EMBL:KOX15221.1};
OS   Saccharothrix sp. NRRL B-16348.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Saccharothrix.
OX   NCBI_TaxID=1415542 {ECO:0000313|EMBL:KOX15221.1, ECO:0000313|Proteomes:UP000037722};
RN   [1] {ECO:0000313|EMBL:KOX15221.1, ECO:0000313|Proteomes:UP000037722}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-16348 {ECO:0000313|EMBL:KOX15221.1,
RC   ECO:0000313|Proteomes:UP000037722};
RA   Noorani M.;
RL   Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic
CC         linkages in type I arabinogalactans.; EC=3.2.1.89;
CC         Evidence={ECO:0000256|ARBA:ARBA00001695,
CC         ECO:0000256|RuleBase:RU361192};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 53 family.
CC       {ECO:0000256|ARBA:ARBA00010687, ECO:0000256|RuleBase:RU361192}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KOX15221.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LGED01000255; KOX15221.1; -; Genomic_DNA.
DR   RefSeq; WP_053722133.1; NZ_LGED01000255.1.
DR   AlphaFoldDB; A0A0M8Y105; -.
DR   STRING; 1415542.ADK67_41890; -.
DR   PATRIC; fig|1415542.3.peg.9035; -.
DR   OrthoDB; 3981930at2; -.
DR   Proteomes; UP000037722; Unassembled WGS sequence.
DR   GO; GO:0031218; F:arabinogalactan endo-1,4-beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015926; F:glucosidase activity; IEA:InterPro.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR011081; Big_4.
DR   InterPro; IPR000421; FA58C.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR011683; Glyco_hydro_53.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR34983; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR   PANTHER; PTHR34983:SF1; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR   Pfam; PF07532; Big_4; 1.
DR   Pfam; PF00754; F5_F8_type_C; 1.
DR   Pfam; PF07745; Glyco_hydro_53; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS50022; FA58C_3; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361192};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361192};
KW   Signal {ECO:0000256|RuleBase:RU361192}.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000256|RuleBase:RU361192"
FT   CHAIN           32..636
FT                   /note="Arabinogalactan endo-beta-1,4-galactanase"
FT                   /evidence="ECO:0000256|RuleBase:RU361192"
FT                   /id="PRO_5039750201"
FT   DOMAIN          29..176
FT                   /note="F5/8 type C"
FT                   /evidence="ECO:0000259|PROSITE:PS50022"
SQ   SEQUENCE   636 AA;  69402 MW;  521E51988825CB24 CRC64;
     MSNRRLTRDL AGLTTLATLV SAGLVAGTAP AGAIFGTADI KPIESNIAAK PWASATAGSG
     SSTAGLAVDG DLTTAWYPDR AGSRQWLTVD LGGTYDNLRK VKVVFPERGV AHRYVVEAST
     DGRRWKTLAD RSHNRAVARG EVHLFTRPAT RFVRLTFTGA PGRARAGVSE LQVFNYLRDD
     LVLGADLSWV DDHQSREYWV NPLSPDKGAG PHQLDVVKDR GMQYARLRVF NEPRSESTGE
     LNAVPRQGPQ RTLTSAQWIK QRNMGLGIDY HYADSWADPS KQPKPLAWAG LEFDELNRAV
     YDFTADHLRR LIRQGTTPDK VAVGNEIING FLYGSEAALI GTTSPPYFVD HADVYQAKPG
     GGLLWKYWGS TDPAEQRLYD QAWDRFTTLA AAGIKAVRDA SPTSKVEVHV IVGTDRLAKT
     MEFWHQYLTR VKAKGQNPDV LAISYYPEWH GTPEALDHNL HTIATTYPDY EIDIAETSYP
     ASGGDGTPMP NSPFPRTIQG QADAIQRVFQ AANDVVDNRG AGVLVWEPAG WQTMFRAVPG
     LANTWEPHAS IDVFNASRAK HILQDTVHTA TAVRTTPKLP SSVQLLTTAN NKITNVPVRW
     QPLPPGATDT PGEVTVTGTT DAGQVTAVID VVPGRG
//