ID A0A0M9UMN7_9MICO Unreviewed; 561 AA.
AC A0A0M9UMN7;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN ORFNames=Y09_3355 {ECO:0000313|EMBL:GAP80494.1};
OS Brachybacterium sp. SW0106-09.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermabacteraceae;
OC Brachybacterium.
OX NCBI_TaxID=1704590 {ECO:0000313|EMBL:GAP80494.1, ECO:0000313|Proteomes:UP000037781};
RN [1] {ECO:0000313|EMBL:GAP80494.1, ECO:0000313|Proteomes:UP000037781}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SW0106-09 {ECO:0000313|EMBL:GAP80494.1,
RC ECO:0000313|Proteomes:UP000037781};
RA Qin Q.L., Li Y., Zhang Y.Z.;
RT "Genome Sequencing of Brachybacterium sp. SW0106-09.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699,
CC ECO:0000256|RuleBase:RU000555};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAP80494.1}.
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DR EMBL; BCAK01000060; GAP80494.1; -; Genomic_DNA.
DR RefSeq; WP_010551998.1; NZ_BCAK01000060.1.
DR AlphaFoldDB; A0A0M9UMN7; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000037781; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:GAP80494.1};
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW ECO:0000256|RuleBase:RU000555};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU000555}.
FT DOMAIN 36..94
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 187..438
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 446..557
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 191
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 480
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 561 AA; 63345 MW; 739118A4CAA0EA58 CRC64;
MTLIDYRRPL SEQRPAAGTS TDPSAPDRST PRGRLEVVGP LGPRFEEILT PEALDFLALL
HDRFASRRTE LLTARTLRRE SIAGGAMFDF LPATRAIRED RSWRVAGPGP GLEDRRVEIT
GPTDRKMTIN ALNSGARVWL ADHEDALSPT WDAVIDGQLN LHDAIRRQID FTGENGKEYR
LAEETATIVF RPRGWHLPEQ HLRWVRADGR SMRASASLVD AGLYLFHNAR ELIERGSGPY
LYLPKLESHL EARLWDEVFA TAERRLGIEH GTIRATVLIE TLPAAFEMEE ILYELRDHCA
GLNAGRWDYL FSIIKMFRDR GRRRVLPDRS QLTMTVPFMR AYTELLVATC HRRGAHAIGG
MSAFIPDRRD PEVTRQALVM VAEDKQREAS DGFDGTWVAH PDLVPAAQAE FDAVLGERPH
QVDRLREDVH VTAEDLLDLE VEGGAITAQG IRQNIRVSLR YLDAWLRGSG AVAIDHLMED
AATVEISRSQ LWQWITQGML TDEGMPVTRE RVEYFIDRAV LELDTEQGSR LPEAAELLKE
AVLGEEFPAF LTNGAYERHL G
//
