ID A0A0M9VKC8_9MICO Unreviewed; 890 AA.
AC A0A0M9VKC8;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=XI38_12870 {ECO:0000313|EMBL:KOS09939.1};
OS Microbacterium chocolatum.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=84292 {ECO:0000313|EMBL:KOS09939.1, ECO:0000313|Proteomes:UP000037737};
RN [1] {ECO:0000313|EMBL:KOS09939.1, ECO:0000313|Proteomes:UP000037737}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SIT 101 {ECO:0000313|EMBL:KOS09939.1,
RC ECO:0000313|Proteomes:UP000037737};
RA Li X., Xu Y.;
RT "Complete genome sequence of Microbacterium chocolatum SIT 101, a bacterium
RT enantioselectively hydrolyzing mesomeric diesters.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOS09939.1}.
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DR EMBL; LAVO01000014; KOS09939.1; -; Genomic_DNA.
DR RefSeq; WP_053548723.1; NZ_KQ440293.1.
DR AlphaFoldDB; A0A0M9VKC8; -.
DR KEGG; mcw:A8L33_12580; -.
DR PATRIC; fig|84292.3.peg.2613; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000037737; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 2.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:KOS09939.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037737}.
FT ACT_SITE 161
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 556
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ SEQUENCE 890 AA; 97263 MW; 3874BEF2CD7BA8A3 CRC64;
MRELTNTEAI DLVGRFEAGQ ETPERMRADV RMLGSLLGKV LRESGSPGLF EDVERLRAAT
IQAYTDETPE AFARAAAIAD SFSVARADEV ARAFTCYFHL VNLAEEHQRV RVLRERDGRP
ERESASDSVA AAFVRLSAEV GDETALERLQ ALRFHPVFTA HPTEARRRAV SSSIRRLASL
LDEHETSRRN GADERRAERR MLEEVDTLWR TAPLRPEKPS PTDEVRAVMA VFDETLYTAI
PHVYRRVDDA LQGPGAGNRA PVVKPFVRIG SWVGGDRDGN PFVTASVTRK AAKIASEHVL
IGLERTAERI GRGLTLDAAS TPPSEALLAL GRRLAAADEE AAGEIAQRSP NEPYRRILLL
IARKLAATRA RDADLAYRDP EHLVADLRVI QDSLVQAGAA RQAYGHLQQL LWQVETYGFH
LAELEVRQHS AVHARVLAEL EAGGERSELA DEVLDVFRSV AFLQERYGPR AAGRYIVSFT
QSAEDLAAVH RLARFAVGPE GTPPVLDVIP LFETFADLQA APGILAEIVE HPEFASRLEA
TGRRLEVMLG YSDSSKDVGP VAANLALYQA QAAISAWARE SGIELTLFHG RGGALGRGGG
PANSAILAQP PHSVDGRFKL TEQGEVIFAR YGDPAIAMRH IDQVAAAILT ASAPSNEERN
RAAADKYAEV AATMDVASRE RFFSLVKAPG FAPWFARVTP MEEIGLLALG SRPARRGLSV
ESLADLRAIP WVFAWTQARI NLAGWFGLGS ALAAVGDEGL LQRAYAEWPL FRTMVDNVAM
SLAKTDDRIA RQYLDLGDRD DLAELVRSEM ALTREWVIRV TGGTELLAGK PVLQRAVKMR
SPYVDALSLL QLRALRALRA ADEGAPVDPE QQRLLLLSVS GVAAGLQNTG
//
