ID A0A0M9X5U7_9ACTN Unreviewed; 475 AA.
AC A0A0M9X5U7;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
GN ORFNames=ADK41_32860 {ECO:0000313|EMBL:KOT30392.1};
OS Streptomyces caelestis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=36816 {ECO:0000313|EMBL:KOT30392.1, ECO:0000313|Proteomes:UP000037773};
RN [1] {ECO:0000313|EMBL:KOT30392.1, ECO:0000313|Proteomes:UP000037773}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-24567 {ECO:0000313|EMBL:KOT30392.1,
RC ECO:0000313|Proteomes:UP000037773};
RA Noorani M.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039,
CC ECO:0000256|RuleBase:RU361241};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771, ECO:0000256|RuleBase:RU361241}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587, ECO:0000256|RuleBase:RU361241}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOT30392.1}.
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DR EMBL; LGCN01000241; KOT30392.1; -; Genomic_DNA.
DR RefSeq; WP_030836196.1; NZ_LGCN01000241.1.
DR AlphaFoldDB; A0A0M9X5U7; -.
DR PATRIC; fig|36816.3.peg.7135; -.
DR OrthoDB; 9810950at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000037773; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU361241};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|RuleBase:RU361241};
KW Reference proteome {ECO:0000313|Proteomes:UP000037773};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU361241}.
FT DOMAIN 6..281
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 324..469
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 475 AA; 50089 MW; E9AA4FB40E5C1301 CRC64;
MTADPLAPLD LAFWNIESAG HPMHLAALGV FAARSPSAAA HAADLLASRA AAVPGLRMRI
RDTWQPLVLR QSLSALRRTR PAPGEPLAAA LRQPLASALR RSLAFGGATR EPDPGFDPLD
HVRLHPPAAD FHTAAGRLMG RPLQRARPPW EAHVLPGEDG LSFAVLFKFH HALADGLRAL
TLAAALMDPM DLPAPRPRPA EPSRGLLPDV REVPGLVRDA LSDAGRALDI GAAVARSTLD
MRSSPALTCA PSGTRRTAGT VIDLDDVHRI RKSAGGTVND VLIAVVAGAL RRWLDERGDG
SEGVAPRALI PVSRRRPRTA HPQGNRLSGY LMRLPVGERD PLARLTQVRT AMDRNKEAGP
HRGAGAVALL ADHVPPLGHR LGGPLLGQAA RLWFDVLVTS VPLPGFGLRL GGDPLGAVFP
LAPLAPGHSL AVAVSTYRGR VHYGLVADGA AVPDLGLLAR ALTEEVAALT AVCDR
//
