ID A0A0M9YKS5_9ACTN Unreviewed; 1177 AA.
AC A0A0M9YKS5;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ADK54_09065 {ECO:0000313|EMBL:KOU51075.1};
OS Streptomyces sp. WM6378.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1415557 {ECO:0000313|EMBL:KOU51075.1, ECO:0000313|Proteomes:UP000037774};
RN [1] {ECO:0000313|Proteomes:UP000037774}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WM6378 {ECO:0000313|Proteomes:UP000037774};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOU51075.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LGDD01000070; KOU51075.1; -; Genomic_DNA.
DR RefSeq; WP_053725035.1; NZ_LGDD01000070.1.
DR AlphaFoldDB; A0A0M9YKS5; -.
DR PATRIC; fig|1415557.3.peg.2018; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000037774; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 642..803
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 828..978
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1177 AA; 129179 MW; E0CA832EE961B5B3 CRC64;
MSLHGLLDAV VRDPALAEAV KAATDGHRMH VDLVGPPAAR PFAIAGLARE AGRPVLAVTA
TGREAEDLAA ALRSMLPAEE IVEYPSWETL PHERLSPRSD TVGRRLAVLR RLTHPSKDDP
SAGPVSVVVA PIRSVLQPQV KGLGDLEPVA LRIGQSADLN TVVEGLAAAA YSRVELVEKR
GEFAVRGGIL DVFPPTEEHP LRVEFWGDDV EEIRYFKVAD QRSLEIAEHG LWAPPCRELL
LTDDVRTRAA ALAEEHPELG ELLGKIAEGI AVEGMESLAP VLVDDMELLI DVLPKGSMAV
VCDPERVRTR AADLVATSQE FLQASWAASA GGGQAPIDVD AASLWGIADV RDRARELGMM
WWSVSPFAAD EADFDEETLK LGMHAPETYR GDTQRALTDT KQWLADGWRT VYVTEGHGPA
SRTVEVLGGE GIAARLAVDL GELTPSVVQV ACGAIDYGFI DPALKLAVLT ETDLTGQRTA
TKDLGRMPAR RRKTIDPLTL EVGDYIVHEQ HGVGRYIEMV QRTVQNATRE YLLVEYAPAK
RGQPGDRLYI PTDQLEQVTK YVGGEAPTLH RLGGADWTKT KARAKKAVKE IAADLIRLYS
ARMAAPGHAF GADTPWQREL EDAFPYAETP DQLSTIAEVK EDMEKTVPMD RLICGDVGYG
KTEIAVRAAF KAVQDGKQVA VLVPTTLLVQ QHFGTFTERY AQFPVNVRAL SRFQSDTEAK
ATLEGLKDGS VDLVIGTHRL FSSETKFKDL GLVIVDEEQR FGVEHKEQLK KLRANVDVLT
MSATPIPRTL EMAVTGIREM STITTPPEER HPVLTFVGPY EEKQIGAAIR RELLREGQVF
YIHNRVESID RAAARLREIV PEARIQTAHG QMGESQLEQV VVDFWEKKFD VLVSTTIVES
GIDISNANTL IVERGDNFGL SQLHQLRGRV GRGRERGYSY FLYPPEKPLT ETAHERLATI
AQHTEMGAGM YVAMKDLEIR GAGNLLGGEQ SGHIAGVGFD LYVRMVGEAV ADYRAQMEGG
EQEEAPLEVK IELPVDAHVP HDYAPGERLR LQAYRAIASA NSEEDIKAVR EELTDRYGKL
PEPVENLLLV AGLRMLARAC GVGEIVLQGP NIRFAPVELR ESQELRLKRL YPRTVIKAPT
RQILVPRPTA GKIGGKPVVG RELLAWTGEF LTTILGS
//
