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Database: UniProt
Entry: A0A0M9YNF1_9ACTN
LinkDB: A0A0M9YNF1_9ACTN
Original site: A0A0M9YNF1_9ACTN 
ID   A0A0M9YNF1_9ACTN        Unreviewed;       549 AA.
AC   A0A0M9YNF1;
DT   09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT   09-DEC-2015, sequence version 1.
DT   08-MAY-2019, entry version 23.
DE   RecName: Full=Urease subunit alpha {ECO:0000256|HAMAP-Rule:MF_01953};
DE            EC=3.5.1.5 {ECO:0000256|HAMAP-Rule:MF_01953};
DE   AltName: Full=Urea amidohydrolase subunit alpha {ECO:0000256|HAMAP-Rule:MF_01953};
GN   Name=ureC {ECO:0000256|HAMAP-Rule:MF_01953};
GN   ORFNames=ADK54_02425 {ECO:0000313|EMBL:KOU54075.1};
OS   Streptomyces sp. WM6378.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1415557 {ECO:0000313|EMBL:KOU54075.1, ECO:0000313|Proteomes:UP000037774};
RN   [1] {ECO:0000313|EMBL:KOU54075.1, ECO:0000313|Proteomes:UP000037774}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WM6378 {ECO:0000313|EMBL:KOU54075.1,
RC   ECO:0000313|Proteomes:UP000037774};
RA   Noorani M.;
RL   Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|HAMAP-Rule:MF_01953,
CC         ECO:0000256|SAAS:SAAS01119912};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|SAAS:SAAS00608819};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1. {ECO:0000256|HAMAP-
CC       Rule:MF_01953, ECO:0000256|SAAS:SAAS00317636}.
CC   -!- SUBUNIT: May form a heterohexamer of 3 UreC (alpha) and 3 UreAB
CC       (gamma/beta) subunits. May also form a heterotrimer of UreA
CC       (gamma), UreB (beta) and UreC (alpha) subunits. Three
CC       heterotrimers associate to form the active enzyme.
CC       {ECO:0000256|HAMAP-Rule:MF_01953}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01953,
CC       ECO:0000256|PROSITE-ProRule:PRU00700,
CC       ECO:0000256|SAAS:SAAS00548017}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|HAMAP-Rule:MF_01953,
CC       ECO:0000256|PIRSR:PIRSR611612-50}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases
CC       superfamily. Urease alpha subunit family. {ECO:0000256|HAMAP-
CC       Rule:MF_01953, ECO:0000256|RuleBase:RU004158,
CC       ECO:0000256|SAAS:SAAS00849550}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01953}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KOU54075.1}.
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DR   EMBL; LGDD01000009; KOU54075.1; -; Genomic_DNA.
DR   RefSeq; WP_053723809.1; NZ_LGDD01000009.1.
DR   EnsemblBacteria; KOU54075; KOU54075; ADK54_02425.
DR   PATRIC; fig|1415557.3.peg.519; -.
DR   OrthoDB; 157757at2; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000037774; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000037774};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01953, ECO:0000256|PROSITE-
KW   ProRule:PRU00700, ECO:0000256|SAAS:SAAS00317631};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01953, ECO:0000256|PROSITE-
KW   ProRule:PRU00700, ECO:0000256|SAAS:SAAS00321417};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01953,
KW   ECO:0000256|SAAS:SAAS00321440};
KW   Nickel {ECO:0000256|HAMAP-Rule:MF_01953,
KW   ECO:0000256|SAAS:SAAS00317628};
KW   Reference proteome {ECO:0000313|Proteomes:UP000037774}.
FT   DOMAIN      127    549       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    304    304       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953, ECO:0000256|PIRSR:PIRSR611612-
FT                                52, ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   METAL       132    132       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953}.
FT   METAL       134    134       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953}.
FT   METAL       212    212       Nickel 1; via carbamate group.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953}.
FT   METAL       212    212       Nickel 2; via carbamate group.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953}.
FT   METAL       344    344       Nickel 1. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953}.
FT   BINDING     214    214       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953, ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     212    212       N6-carboxylysine. {ECO:0000256|HAMAP-
FT                                Rule:MF_01953, ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
SQ   SEQUENCE   549 AA;  56979 MW;  3DC2909A15639898 CRC64;
     MDPYEYASVH GPRTGDRIRL GDSGLTVRVE SDSQAPGDEF LAGFGKTARD GLHLKAAAVR
     DTCDVVISNV VVIDAVQGIR KVSIGIREGR ICSIGRAGNP DTLDGVDVVV GTGTSIVSGE
     GLIATAGAVD THVHLLSPRI MEASLASGVT TIIGQEFGPV WGVGVNSPWA LRGAFAAFDA
     WPVNIGFLGR GSSSDGAPLV EALAEGGACG FKVHEDMGAH TRALDTALRV AEEHDVQVAL
     EDTLKVLEGR TIHAFHIEGC GGGHVPNVLK MAGVPNVIGS STNPTLPFGR DAVAEHYGMI
     VSVHDLKTDL PGDAAMARDR IRAGTMGGED VLHDLGAIGI TSSDAQGMGR AGETVRRTFA
     MAGKMKAELG PLEGDGPGDD NARVLRYMAK LTINPAIAHG LSHEIGSIET GKMADIVLWR
     PAYFGAKPQM VLKSGFPAWG VTGDPNAATD TCEPLVLGPL FGAHGGTAAE LSVAFVAKAA
     LDQGGDQLPT RRRRVAVRGT RGIGPADLLL NSRTGAVGVD GRTGLVTLDG DPLRSEPADS
     VSLNRLYFL
//
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