ID A0A0N0A9U6_9ACTN Unreviewed; 500 AA.
AC A0A0N0A9U6;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Dioxygenase {ECO:0000256|RuleBase:RU364048};
DE EC=1.13.11.- {ECO:0000256|RuleBase:RU364048};
GN ORFNames=ADL04_17140 {ECO:0000313|EMBL:KOV96595.1};
OS Streptomyces sp. NRRL B-3648.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1519493 {ECO:0000313|EMBL:KOV96595.1, ECO:0000313|Proteomes:UP000037702};
RN [1] {ECO:0000313|EMBL:KOV96595.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-3648 {ECO:0000313|EMBL:KOV96595.1};
RA Noorani M.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1,
CC ECO:0000256|RuleBase:RU364048};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1,
CC ECO:0000256|RuleBase:RU364048};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787, ECO:0000256|RuleBase:RU364048}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOV96595.1}.
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DR EMBL; LGDZ01000182; KOV96595.1; -; Genomic_DNA.
DR RefSeq; WP_053709400.1; NZ_LGDZ01000182.1.
DR AlphaFoldDB; A0A0N0A9U6; -.
DR PATRIC; fig|1519493.3.peg.3657; -.
DR OrthoDB; 6636843at2; -.
DR Proteomes; UP000037702; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR InterPro; IPR006311; TAT_signal.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR Pfam; PF03055; RPE65; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|RuleBase:RU364048, ECO:0000313|EMBL:KOV96595.1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU364048};
KW Reference proteome {ECO:0000313|Proteomes:UP000037702}.
FT BINDING 191
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 239
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 302
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 493
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 500 AA; 54876 MW; DCB3C1E93AF0576C CRC64;
MAEHTRRTVL RGTAGLAAAG ALAGTAGRPA AAARGTSAPA PRHLPFLEGA FAPVTEELTA
YDLPVTGRIP RELNGRLLRN GPNVLGLEDP RAHHWMLGEG MVHGVRLRDG RAEWYRNRWV
RSAQVARKLG EPYPGPVPPD DFACNTHVIP YRGRILALQE SGPAPYELDG ELNTVGPYDF
RGTLEGAFTA HTKFDAHAGE LHALAYYPAW DHIRHLVVDP SGRVARTTRI PMPDAPMIHD
FALTARHVVV IDIPVTFDPA AARRGDPVPY LWNPRHPARV GVMPRSGGPV RWFPIDPVFY
SHTLNAYDEG ASVVVDLTTY PAPFEVAGRG SGGPHAVGTP RLERWTVEVE RGRVRQRVLD
DRPQEFPRVN EALVSRRHRY GYSAACAEFT LAYLSPDGNP PDRAFSNALI KHDLLRGTTR
LHRLPRGAAA GEAVFVPRDP ADHRAAEDDG YALAYVYDPD RRATDLVILA AQDFTGEPVA
RVHLPGRVPL GFHGSWIPDA
//
