ID A0A0N0J627_9PROT Unreviewed; 939 AA.
AC A0A0N0J627;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=IP80_20125 {ECO:0000313|EMBL:KPF42797.1};
OS beta proteobacterium AAP65.
OC Bacteria; Pseudomonadota; Betaproteobacteria.
OX NCBI_TaxID=1523424 {ECO:0000313|EMBL:KPF42797.1, ECO:0000313|Proteomes:UP000037849};
RN [1] {ECO:0000313|EMBL:KPF42797.1, ECO:0000313|Proteomes:UP000037849}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AAP65 {ECO:0000313|EMBL:KPF42797.1,
RC ECO:0000313|Proteomes:UP000037849};
RA Zeng Y., Feng F., Liu Y., Koblizek M.;
RT "Novel Diversity of Limnic Aerobic Anoxygenic Phototrophic Bacteria as
RT Revealed by High-throughput Strain Identification and Genome Sequencing.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPF42797.1}.
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DR EMBL; LJHW01000054; KPF42797.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0J627; -.
DR STRING; 1523424.IP80_20125; -.
DR PATRIC; fig|1523424.3.peg.3527; -.
DR Proteomes; UP000037849; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:KPF42797.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037849}.
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 160
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 589
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 939 AA; 103844 MW; DC9B543D84708490 CRC64;
MKTASASTRT RTKTASDDAA AKNKPLVEDI RLLGRILGDV IREQEGTAAF ELIERVRQLS
VAYRLKADAS AGRVLDRLLK NLSADQTVTV IRAFSYFSHL ANIAEDRHHV RRRSVHERAG
DLQEGSLALS FERLQRADHR AADIAATLHG AYISPVLTAH PTEVQRKSIL DAERAVAELL
EQRDELPTEE RRAENEALMR ARVTQLWQTR MLRTAKLTVA DEIENALSYY GTTFLREIPR
LYRDVERALP GHAVGNFLRM GHWIGGDRDG NPNVTAATLA HALKRQAEVA LRFYLTEVHE
LGAELSISGT LAPVSPEMQA LAAASPDSNP HRLDEPYRRA LIGMYSRLAA TLQALTGTEA
LRHAVAPQNP YPGPEAFLAD LRTIERSLGS HHAQALVGPR LAPLMRAVQV FGFHLATLDL
RQSSDKHEAV VAELLRVARI EADYSALSEP QRRTCLLALL NDARPLRVHG SPYSPLALDE
LAIFETAREA LTRYGAQTLR HCIISHTEEV SDLLEVLLLQ KEAGLLTGTL DQPGTKAALI
TVPLFETIGD LRRSEPIMRE FYALPGVHAL VRNSGAEQDV MLGYSDSNKD GGSFTSHWEL
FRAETALAAL FAELNAAQAR SKPITLRLFH GRGGTVGRGG GPSYQAILAQ PPGTVNGQIR
LTEQGEVIAS KYANPEIGRR NLETLVAATL EATMLHPTKP APKAFLDAAA EISEASFQAY
RQLVYGTPGF TDYFFAATPI REIAELNIGS RPASRKATRA IEDLRAIPWS FSWGQCRMAL
PGWCGFGSAI EAFLAQGDRA ERAALLQRMH KQWPFFRTLL SNLDMVLAKS DLRIAARYVE
LVEDKKVAKR IFGLLKAEWQ RASEALTLIT GQQQPLQGNP ALARSIAHRF PYLDPLNHLQ
VELMRRYRTR REGQEGVERL QRGIHLSING IAAGLRNTG
//
