ID A0A0N0MEW7_9PROT Unreviewed; 446 AA.
AC A0A0N0MEW7;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:KPH86936.1};
GN ORFNames=GLUCOINTEAF2_0202412 {ECO:0000313|EMBL:KPH86936.1};
OS Komagataeibacter intermedius AF2.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Komagataeibacter.
OX NCBI_TaxID=1458464 {ECO:0000313|EMBL:KPH86936.1, ECO:0000313|Proteomes:UP000031553};
RN [1] {ECO:0000313|EMBL:KPH86936.1, ECO:0000313|Proteomes:UP000031553}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF2 {ECO:0000313|EMBL:KPH86936.1,
RC ECO:0000313|Proteomes:UP000031553};
RA Santos R.A., Berretta A.A., Barud H.S., Ribeiro S.J., Gonzalez-Garcia L.N.,
RA Zucchi T.D., Goldman G.H., Riano-Pachon D.M.;
RT "Draft Genome Sequence of Komagataeibacter intermedius Strain AF2, Isolated
RT from Kombucha Tea.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPH86936.1}.
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DR EMBL; JUFX02000179; KPH86936.1; -; Genomic_DNA.
DR RefSeq; WP_039735263.1; NZ_JUFX02000179.1.
DR AlphaFoldDB; A0A0N0MEW7; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000031553; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 54..139
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 165..384
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 446 AA; 51629 MW; F6D1A1F6998E82FD CRC64;
MSKTDLKLLI IQPSHYRSKT DRTIFKTRRR QMVPLALPYL AALTPREWTV TLLDEQLEDI
DFDVPVDVVA LSAWTLHSPR AYDIAAEFRR RGVKVIMGGP HAFFYADEAA EHCDAVGVGE
AEPIWKTMLE DAAADRLQKI YRAAPLKELN DLPPPRYDLL DLKKFGPFRT FAVQSSRGCP
FVCDFCSERF YLGGRYRWRP VDQMVAELER IKNKGSHFFF GESNFGGKKS RAMELMEGLV
PLKIRWSTLW SSNLCLDTGF LDLAQRSGVM HVNIGIESID EDMLQGMKKG WNKASRYGEM
FDNLRRRDIS YSLNFIFGYD DEHPDVYNAT LSFLEAHKVP AAYFNILTPT KGTALFERMK
KAGRIIDPED IDRWPGQVCH IWPKNCTPGQ MEQRIQTMYR KFYSMRSMVH RLPFPRTRSD
MSSWILNLTE RRMAFSSTGN NDFDIY
//
