ID A0A0N0MM31_9ACTN Unreviewed; 726 AA.
AC A0A0N0MM31;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:KPH99270.1};
DE EC=3.2.1.68 {ECO:0000313|EMBL:KPH99270.1};
GN ORFNames=OK074_6082 {ECO:0000313|EMBL:KPH99270.1};
OS Actinobacteria bacterium OK074.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592327 {ECO:0000313|EMBL:KPH99270.1, ECO:0000313|Proteomes:UP000037991};
RN [1] {ECO:0000313|EMBL:KPH99270.1, ECO:0000313|Proteomes:UP000037991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK074 {ECO:0000313|EMBL:KPH99270.1,
RC ECO:0000313|Proteomes:UP000037991};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPH99270.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LJCV01000299; KPH99270.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0MM31; -.
DR PATRIC; fig|1592327.3.peg.8959; -.
DR OrthoDB; 3236218at2; -.
DR Proteomes; UP000037991; Unassembled WGS sequence.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:KPH99270.1};
KW Hydrolase {ECO:0000313|EMBL:KPH99270.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037991}.
FT DOMAIN 177..589
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 466..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..575
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 559..575
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 726 AA; 81382 MW; 43BC10E289A2B8E9 CRC64;
MQVWPGEAYP LGATYDGAGT NFAVFSEAAH RIELCLLHDD GSETAVELRE TDAFVRHAYL
PGVMPGQRYG FRVHGPYAPE RGLRCNSAKL LLDPYARAVS GSIGWGEEVY GYHFDAPERR
NDLDSAPHTM SSVVVNPYFD WGDDRRPRTE YHHTVLYEAH VKGLTMRHPE LPEELRGTYA
ALAHPAIIEH LTKLGVTALE LMPVHQFVND HRLADMGLSN YWGYNTIGFF APHNAYASWG
DRGQQVLEFK SAVRALHEAG IEVILDVVYN HTAEGNHLGP TLSFRGIDNP SYYRLADDPR
YYTDTTGTGN SLLMRSPHVL QLIMDSLRYW VTEMHVDGFR FDLAATLARQ FHEVDRLSSF
FDLVQQDPVV SQVKLIAEPW DVGEGGYQVG NFPPLWTEWN GKYRDTVRDL WRGEPRTLAE
FASRLTGSSD LYQDDGRRPL ASINFVTCHD GFTLHDLVAY NDKHNDANGE GNRDGESHNR
SWNCGVEGDS DDPAVLALRG RQMRNFVATL MLSQGVPMLS HGDEFARSQG GNNNAYCQDS
ELAWVKWPDP GPAPDSDPDQ DSGSGSGSDD GSSSSDQLLA FTRAMVWLRR DHPVFRRRRF
FHGRPVEGTH DDLSDIAWFT PEGQEMTQRD WDSAQASALT VFLNGNAISE PDARGERIAD
DSFLLMFNAF HEPLEFVVPV DHGRQWQVIV DTSREEGVPP GTGAKVQAGE RLTLTDRSMT
VLQRPA
//
