ID A0A0N0MP85_9ACTN Unreviewed; 689 AA.
AC A0A0N0MP85;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Isoamylase {ECO:0000313|EMBL:KPI01738.1};
DE EC=3.2.1.68 {ECO:0000313|EMBL:KPI01738.1};
GN ORFNames=OK006_7927 {ECO:0000313|EMBL:KPI01738.1};
OS Actinobacteria bacterium OK006.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592326 {ECO:0000313|EMBL:KPI01738.1, ECO:0000313|Proteomes:UP000037912};
RN [1] {ECO:0000313|EMBL:KPI01738.1, ECO:0000313|Proteomes:UP000037912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK006 {ECO:0000313|EMBL:KPI01738.1,
RC ECO:0000313|Proteomes:UP000037912};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four Actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI01738.1}.
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DR EMBL; LJCU01000257; KPI01738.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0MP85; -.
DR SMR; A0A0N0MP85; -.
DR PATRIC; fig|1592326.3.peg.9846; -.
DR Proteomes; UP000037912; Unassembled WGS sequence.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Glycosidase {ECO:0000313|EMBL:KPI01738.1};
KW Hydrolase {ECO:0000313|EMBL:KPI01738.1};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 151..561
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 689 AA; 75370 MW; 37966A02108CDCA2 CRC64;
MTTTTPQSIA PRRHWSTTPG RARPPGASIT PDGVNFAVHA AGARGMRLVL MRAADGTAEA
ELPFPDGGRF GTVFTMTVPD LDPDRFHYGY RVEYASGELS PILLDPYAKE LAGAGNWGER
PQYRSAVLAD DFGWGEDRSP RIPQNELIVY EVHVRGYTRH ASSEVAQPGT YAGLREKIPY
LQSLGVNCVE LLPVFEFDET DNTYMSPETG EPLRNFWGYN SVGFFAPKAS YAADATPGGT
ARELKTLVKA LHAAGIAVIL DVVYNHTAEG DHRGPTQSLR GLDESAYYLL DARGRPVNLT
ATGNTVNCNH PVTRELILDS LRYWATEYRV DGFRFDMAAI LTRGTHGEPL PDPPLVEAIA
RDPVLAHCTL IAEASDAAGS YQVGSFPGYG RWSEWNMRYR DAIRRLLLGR PGSVAEFATR
LVGSPDLYAG RGATPSVNYI TCHDGLTLAD WTSYDRRHNE ANGEDGRDGI PDEESWNCGH
EGPTDDPAIL RLRAQQERNA LLLLFTSQGV PMLLAGDEFG RTQHGNNNAY GQDGPIGWVD
WGMLGTQARL ARFTRRCIAL RKAHPVLRRA AHPDGTVRAG VPYPAVSWHG EQPWQPDWSP
SSTLLAAVLY QERTDGPPDC VYLAVNTGDS ERTVQVPAPP RGLAWHVFAD TGSSADLDGH
EAGEEPLCTD IHRRLAARST VLLVGQSHG
//
