ID A0A0N0MRB3_9ACTN Unreviewed; 942 AA.
AC A0A0N0MRB3;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=OK074_4748 {ECO:0000313|EMBL:KPI04221.1};
OS Actinobacteria bacterium OK074.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592327 {ECO:0000313|EMBL:KPI04221.1, ECO:0000313|Proteomes:UP000037991};
RN [1] {ECO:0000313|EMBL:KPI04221.1, ECO:0000313|Proteomes:UP000037991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK074 {ECO:0000313|EMBL:KPI04221.1,
RC ECO:0000313|Proteomes:UP000037991};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI04221.1}.
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DR EMBL; LJCV01000269; KPI04221.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0MRB3; -.
DR PATRIC; fig|1592327.3.peg.7249; -.
DR Proteomes; UP000037991; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:KPI04221.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037991}.
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 168
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 600
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 942 AA; 104077 MW; E0A0FEAF191087FD CRC64;
MSSADDTKTT PGAPTASGDP TATTHGQATG TPHGTAPAAP EATSSALRAD IRRLGDLLGE
TLVRQEGPEL LELVEKVRRL TREDGEAAAE LLRGTELETA AKLVRAFSTY FHLANVTEQV
HRGRELRARR AAEGGLLART ADRLKDADPD HLRETVQHLN VRPVFTAHPT EAARRSVLNK
LRRIAALLDT PVIESDRRRY DTRLAENIDL VWQTDELRVV RPEPADEARN AVYYLDELHA
GAVGDVLEDL TAELERVGVK LPDETRPVTF GTWIGGDRDG NPNVTPQVTW DVLILQHEHG
INDALDLIDE LRGFLSNSIR YTGATEELLE SLRADLEQLP GISPRYKRLN AEEPYRLKAT
CIRQKLVNTK QRLAKGTPHE PGRDYLGTAE LLRDLTLIQT SLREHRGGLF ADGRMNRTIR
TLAAFGLQLA TMDVREHADA HHHALGQLFD RLGEESWRYE DMPREYRHKL LAKELRSRRP
LAPTPAPLDA AGTKTIGVFE TVKRALAVFG PEVIESYIIS MCQGADDVFA AAVLAREAGL
IDLHAGWAQI GIVPLLETTD ELKAADTILE DMLSDPSYRR LVALRGDVQE VMLGYSDSSK
FGGITTSQWE IHRAQRRLRD VAHRYGVRLR LFHGRGGTVG RGGGPSHDAI LAQPWGTLEG
EIKVTEQGEV ISDKYLIPAL ARENLELTVA ATLQASALHT SPRQSVEALA RWDAAMDVVS
EAAHSAYRGL VEDPDLPTYF LASTPVDQLA ELHLGSRPSR RPGSGVSLDG LRAIPWVFGW
TQSRQIVPGW FGVGSGLKAL REAGLDTVLD EMHEQWHFFR NFVSNVEMTL AKTDLRIAQH
YVDTLVPENL KHVFDAIRTE HELTVAEILR VTGEAELLDA QPVLKQTFTI RDAYLDPISY
LQVALLKRQR DAATAGVEAD PILARALLLT VNGVAAGLRN TG
//
