ID A0A0N0NDU4_9ACTN Unreviewed; 283 AA.
AC A0A0N0NDU4;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=2,5-didehydrogluconate reductase {ECO:0000313|EMBL:KPI30298.1};
DE EC=1.1.1.274 {ECO:0000313|EMBL:KPI30298.1};
GN ORFNames=OK074_6735 {ECO:0000313|EMBL:KPI30298.1};
OS Actinobacteria bacterium OK074.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592327 {ECO:0000313|EMBL:KPI30298.1, ECO:0000313|Proteomes:UP000037991};
RN [1] {ECO:0000313|EMBL:KPI30298.1, ECO:0000313|Proteomes:UP000037991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK074 {ECO:0000313|EMBL:KPI30298.1,
RC ECO:0000313|Proteomes:UP000037991};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI30298.1}.
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DR EMBL; LJCV01000003; KPI30298.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0NDU4; -.
DR PATRIC; fig|1592327.3.peg.296; -.
DR OrthoDB; 9804790at2; -.
DR Proteomes; UP000037991; Unassembled WGS sequence.
DR GO; GO:0050580; F:2,5-didehydrogluconate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19133; AKR_AKR5F1; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 2.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:KPI30298.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037991}.
FT DOMAIN 21..257
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 48
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 73
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 283 AA; 31758 MW; C85A48AE1FF65AF1 CRC64;
MQTVTLNNGV EMPILGFGVF QIPLEETEQA VTDALAAGYR LLDTAASYGN EEAVGRAIKN
SGIPRGDLFV TTKLYVQDAP AERNTKRAFE TSLTKLGLDH LDLYLMHQPY GDVYGQWRAM
QDLNRTGLAK AIGVANFYPD RLLDLIFNNE ITPAVNQIET HPFFQRADYQ TLMREHGVQI
QSWGGFAEGK NNLFTNPVLS EIAAKHGKSV AQVVLRWLTQ RDVIAIPKSV RPERMAENLD
VFDFELTDDQ LATIATLDTG ASLFFDHHDP EMVTWLSKRR LDS
//
