ID A0A0N0TV74_9ACTN Unreviewed; 1163 AA.
AC A0A0N0TV74;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=ADL06_04310 {ECO:0000313|EMBL:KOX36769.1};
OS Streptomyces sp. NRRL F-6491.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1519495 {ECO:0000313|EMBL:KOX36769.1, ECO:0000313|Proteomes:UP000037743};
RN [1] {ECO:0000313|EMBL:KOX36769.1, ECO:0000313|Proteomes:UP000037743}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL F-6491 {ECO:0000313|EMBL:KOX36769.1,
RC ECO:0000313|Proteomes:UP000037743};
RA Noorani M.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOX36769.1}.
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DR EMBL; LGEE01000022; KOX36769.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N0TV74; -.
DR PATRIC; fig|1519495.3.peg.909; -.
DR Proteomes; UP000037743; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..58
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT REGION 63..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1163 AA; 129051 MW; 3E733EC46E89D928 CRC64;
MLDGAARLKD MFDACNEMGM THIAMSDHGN LHGAYDFFHT AKKAGVTPII GIEAYVAPES
RRNKRKIQWG QPHQKRDDVS GSGGYTHKTI WAANATGLHN LFRLSSDAYA EGWLQKWPRM
DKETISQWSE GLIASTGCPS GELQTRLRLG QFDEALKAAS EYQDIFGKDR YFLELMDHGI
EIEHRVRDGL LEIGKKLGIP PLVTNDSHYT YAHEATAHDA LLCIQTGKNL SDPDRFRFDG
TGYYLKSTDE MYAVDSSDAW QEGCRNTLLV AEQIDTSGMF EAKNLMPRFD IPDGYTEVTW
FREETMRGMA RRFPGGIPED RMKQVEYEMD TIISMGFPGY FLVVADFIMW AKKQGIAVGP
GRGSAAGSIV AYAMGITDLD PIPHGLIFER FLNPERISMP DVDIDFDERR RVEVIRYVTE
KYGSDKVAMI GTYGKIKAKN AIKDSARVLG YPYAMGDRLT KAMPADVLGK GIDLDGITNP
SHPRYSEAGE IRAMYENEPD VKKVIDTARG VEGLVRQMGV HAAGVIMSSE PIVDHAPIWV
RHTDGVTITQ WDYPQCESLG LLKMDFLGLR NLTIMDDAVK MVKANKGVDL DLLALPLDDP
KTFELLQRGD TLGVFQFDGG PMRSLLRLMK PDNFEDISAV SALYRPGPMG MNSHTNYALR
KNKQQEITPI HPELEKPLEE VLAVTYGLIV YQEQVQKAAQ IIAGYSLGEA DILRRVMGKK
KPEELAKNFT IFQAGARKNG YSDEAIQSLW DVLVPFAGYA FNKAHSAAYG LVSYWTAYLK
ANHPAEYMAG LLTSVKDDKD KSAVYLNECR RMGIKVLPPN VNESQSNFAA QGDDVILFGL
TAVRNVGTNV VESIIKTRKS KGKYSSFPDF LDKVEAVVCN KRTVESLIKA GAFDEMGHTR
KGLVAHHEPM IDNVVAVKRK EAEGQFDLFG GMGEETNDEP GFGLDVEFSD VEWEKSYLLA
QEREMLGLYV SDHPLFGIEH VLSDKTDAAI SQLTGGEHSD GAVVTIGGII SGLQRKMTKQ
GNAWAIATVE DLAGSIECMF FPATYQLVST QLVEDTVVFV KGRLDKREDV PRLVAMELQV
PDLSNAGTNA PVILTIPTVK VTPPMVSRLG EILKHHKGNT EVRIRLQGPR KTTVLRLDRH
RVTADPALFG DLKVLLGPSC LAG
//
