ID A0A0N1GRF2_9ACTN Unreviewed; 1197 AA.
AC A0A0N1GRF2;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=OV320_7337 {ECO:0000313|EMBL:KPI25862.1};
OS Actinobacteria bacterium OV320.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592329 {ECO:0000313|EMBL:KPI25862.1, ECO:0000313|Proteomes:UP000037870};
RN [1] {ECO:0000313|EMBL:KPI25862.1, ECO:0000313|Proteomes:UP000037870}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OV320 {ECO:0000313|EMBL:KPI25862.1,
RC ECO:0000313|Proteomes:UP000037870};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI25862.1}.
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DR EMBL; LJCX01000029; KPI25862.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N1GRF2; -.
DR PATRIC; fig|1592329.3.peg.6712; -.
DR Proteomes; UP000037870; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000037870}.
FT DOMAIN 17..343
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 344..666
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 835..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1164..1197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 856..891
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1197 AA; 129624 MW; AAA7E73C77AC9F60 CRC64;
MPARISDPEQ LKELLGIPFT PEQTACITAP PAPQVIVAGA GSGKTTVMAA RVVWLVGTGQ
VAPEQVLGLT FTNKAAGELA ERVRKALVKA GVTDPDLIDP DNPPGEPVIS TYHSFAGRLL
TDHGLRIGLE PTSRLLADAT RYQLAARVLR EAPGPYPSLT RSFADLISDL LALDAELAEH
LVRPEDLRAW DAALLNGLEG AGLTNADLRK VPEAAAARRE LAELVLRYRT AKRERDLLDF
GDQIALAARL SQVPEVGPIL REEFRVVLLD EYQDTSVAQR ILLAGLFGGG TGHPVTAVGD
PCQAIYGWRG ASVANLDDFP EHFAHTDGSR ARRQSLSENR RSGGRLLDLA NGLAEPLRAM
HAGVEALRPA PGAERDGLVR CALLNTHAEE MDWIADSIAH LVRTGKAPGE IAVLCRTATD
FAEIQGALVA RDIPVEVVGL SGLLHLPEVA DLVAVCEVLQ DPGANASLVR LLTGPRWRIG
PRDLALLGRR ARLLVTHARG GGDDDPDRRL AEAVEGVDPS EVISLADALD TFLETPLYGD
GGAGYDDGLP FSPDARVRFA RLATELRDLR RSLADPLMDV LHRVLAVTGL EVELSASPQA
LAARRRETLS NFLDVAASFA ASESQASLLA FLGFLRTAAQ YEKGLDNALP GGENTVKVLT
AHKSKGLEWD VVAVPGLVTG TFPSGQGREK WTAQAKVLPH ELRGDAATLP DLDSWDARGM
KAFHEAMKDH QHTEELRLGY VTFTRPRSLL LGSGHWWGPT QKKPRGPSDF LQELYRHCAA
GHGEIEAWAD EPEEGTENPV LHRPTADQVW PLPLDDDSLA RRRAAAETVL AHLEDLASHQ
DGNPGATHDP DTYDDPDWPP PPDEDELSYG SEYDEPEYGD PEYDEPEYGG PGYDDPEYGS
DSSYDESGPF EDAPLENTPL AVPHQATAPE LPVTRPRRRP SLTPEEARAV ASWDRDLDAL
AGELLRARRS VTDVTLPTTL TASQVLRLAA DPDGLAQELA RPMPRPPQPA ARRGTRFHAW
VEARFEELTL PLLEPHELPG EDAEIADERD LEALKEAFES TEYAHRTPYR VEAPFQLALA
GRVVRGRIDA VYKEGDGDTA TYDIVDWKTH RARTADPLQL ALYRLAWAEQ QGVPVESVTA
AFLYVRSGDV VRPEGLPDRA ALERLLTGED GAGDGRGGPA AQVNPAQGPG EDVGAGR
//
