ID A0A0N1NF99_9ACTN Unreviewed; 4663 AA.
AC A0A0N1NF99;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE SubName: Full=Amino acid adenylation domain protein {ECO:0000313|EMBL:KPI05694.1};
DE EC=5.1.1.13 {ECO:0000313|EMBL:KPI05694.1};
GN ORFNames=OK074_4409 {ECO:0000313|EMBL:KPI05694.1};
OS Actinobacteria bacterium OK074.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1592327 {ECO:0000313|EMBL:KPI05694.1, ECO:0000313|Proteomes:UP000037991};
RN [1] {ECO:0000313|EMBL:KPI05694.1, ECO:0000313|Proteomes:UP000037991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK074 {ECO:0000313|EMBL:KPI05694.1,
RC ECO:0000313|Proteomes:UP000037991};
RA Brown S.D., Utturkar S.M., Klingeman D.M., Pelletier D.;
RT "Draft genome sequences for four actinobacteria strains OK006 OK074 OV450
RT and OV320.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI05694.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LJCV01000261; KPI05694.1; -; Genomic_DNA.
DR PATRIC; fig|1592327.3.peg.6791; -.
DR OrthoDB; 2472181at2; -.
DR Proteomes; UP000037991; Unassembled WGS sequence.
DR GO; GO:0047689; F:aspartate racemase activity; IEA:UniProtKB-EC.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR CDD; cd12117; A_NRPS_Srf_like; 1.
DR CDD; cd19543; DCL_NRPS; 3.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 3.40.50.980; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 3.
DR NCBIfam; TIGR01720; NRPS-para261; 3.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 3.
DR Pfam; PF13193; AMP-binding_C; 3.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 12.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Isomerase {ECO:0000313|EMBL:KPI05694.1};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000037991}.
FT DOMAIN 966..1042
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2518..2592
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4089..4163
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1424..1448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1424..1446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4663 AA; 491575 MW; 5A66EC27D6FD1665 CRC64;
MTRSLVEDVW PLSPLQEGLL FHAAFDDQGP DVYQGQRTLE LAGPLDAGRL RTSWEALLAR
HGALRASFRG RKSGEAVQVI AREATLPWRM EDVSGLAEAD ALAEAERLSA RERSERFDLA
VAPLLRLLLI RLGKDRHHLV ITSHHVVMDG WSMAVLFNEL PAVYAAGGDT RGLPRTTSYR
DYLVWLGRQD KETARTAWRA ELAGADEPTL VAPADPARTP VAPESLITRL SAELTHGLTQ
LARGQGLTVN TVIQGAWALL LARLAGRTDV VFGATVAGRP AELPGVESMV GLFINTLPVR
VNLDGGQSVA EMLTRLQQRQ AALMAHQHLG LSEVQKAAGP GAVFDTIVIY ENYPRPPQEE
PAPDTFAMRP LGEAEAAHYP LTLVVAPEGE RMAVKLDHRP DLFDHTAAQS VVERLVRVLE
QVVADPGMLV GRIDLLDGLD GVERELVVEG WNATAVPVAG VSVPELFAGQ VERSPGVVAV
VDGERALTYG ELAGEAGRFA VYLRGLGVGR GDRVAVVMER SADLLVVLLG VWLAGAAYVP
VDVEYPVERV GLLLADSGPV AVVCTESTRG VVSGGTAVRL VVLDDAGVVA EIAGCRADGF
GVPVGSGDLA YVMYTSGSTG VPKGVAVPHG SVAGLVGDRG WSVGAGDAVL MHAPHAFDVS
LFEVWVALVS GGRVVVAEPG VVDAGRIREA VASGVTAVHV TAGLFRVLAE ESPECFGGLR
EVLTGGDVVP VGSVERVREA CPEVSVRHLY GPTEITLCAT WHVLVPGATG GPVLPIGRPL
SNRQVYVLDA FLRPVPAGVV GELYVAGSGL ARGYLRRPGL TAERFVACPF VGGGRMYRTG
DLARWSAEGE LLFVGRADEQ VKVRGFRVEL GEVEAVLAGH VSVSQAVVVA RQDGPGDKRL
IGYVVPEKEE DADPQEIRAY VAAQLPEYMV PAAVVVLDAL PLTVNGKVDR AALPAPDFAE
RVTGREPRTA VERVLCDVFA GVLGLERVGV EDGFFELGGD SISSMQLASR ARRAGLVVTP
RQVFEEKTPE RLAVVAEVVG KAATTHDVGV GEVPWTPVMR ALGERAARPG FAQWVILGAP
AGLGLETLAG GLSAVLDAHD MLRARTVPGE PKLIVGERGS VDVARLVVRV DADADSSPAD
LDETAGRAAQ EAVKRLDPGS GVMVQAVWVD AGPGRVGRLV FVVHHLVVDG VSWRVLVPDL
QGACEAVAAG HVPVLDAVGT SFRRWAQVLA VEAAGERRVA ELGDWLALLG EGQLPLGRRE
LDPALDTVRT LRSQSWVVPP EQAATLLART PNAFHCGVDD VLLAALAGAV AHWQPHAAPG
LLVDVEGHGR EPLGGVDLSR TVGWFTSAHP VRLDVAGAEL SQVVAGGPAA GRLLKAVKEQ
ARAVPGDGLG YELLRHLNPE TGPALRAAPT PQIGFNYMGR FTSGGTATTS TGTSTSTSTG
TGTGQPKAAP WQLAGHTAIG GSVDPATPLP HALEANVTVL DTPDGPELTL TLSWPGQLLE
DADAERLGRT WAEMLGGLAV HTAAPGSGGH TPSDFPLLDG VLTQGGVERL EETVAGLADI
WPLSPLQEGI LFHATFDDGA PDVYQSQRVL AVDGPLDAAR LRAAWQAVLV RHEALRASFH
RLGTGDAVQV VARDVELPWR EADLSDRTEA GALVEAERLA AAEAAERIDL AVAPLLRLLL
IRLGADRHRL VVTSHHIVAD GWSTPILLNE VSEAYAASGA PSLPGRTASY ADYLTWLDRQ
DKEAARDAWR VELAGADEPT LVAPADLSGA LVLPEERSVR LSRRATRAVT ELARAQGLTV
NTVVQGAWAL VLARLARRTD VVFGTTVAGR PPEVPGVESM IGLFINTLPV RVRLDARQPV
LAMLTQLQRR RTALMVHQHT GLAEIQAVGG AGAVFDTILM FENYPRSMPA LPGPDRAGAR
VALTQVDSQV GTHYPLALGV VPEERLEVRV AYRPDVFDGR RATQVGRQLL RVLEQLAADP
ALRVGDVDVL EAADRSSVVE RWNATDRPVP TGTLAELFEA QARRSPDAVA VIAHDRQWSY
AELGRSADRV AHELLTRGVR RGDLVGVVME RSADVVAVLL GVAKAGAGFV PVDPAYPAER
IGHVLADAGP ALVVCTPTTV PVVPAGVARW VYGDRGTVTV LTGRTPRPAD AGSRVTDPAY
VIYTSGSTGT PKGVVVTHGG IGNLATGQIE RFAVRADSRV LQLASWSFDA AVSEMCMALL
SGAALVVVDG DRLPPHGSLA EVAAEFGVTH LTVPPSVLAT VDTLPDAVET LVVAGEDCPP
WLAERWSGRR LVNAYGPTEV TVCAAMSEPL RPAGDGPLPV GPVPVGRPLV NTRAYVLDDF
LKPVAPGTVG ELYVVGPGLA RGYLHRPRLT AERFVASPFR GPGDAGRMYR TGDLVRWSAG
GELLFVGRAD EQVKVRGFRV ELGEVEAVLA GHASVSQAVV VARRDGPGDK RLIGYVVPEG
EVDVDARELR AYAADRLPEY MVPAAVLVLD ALPLTVNGKV DRAALPAPDF AARVSGREPR
TDTEATLCGL FAEVLGLERV GAEDGFFELG GDSISSMQLA SRARGAGLVV TPRQVFEEKT
PERLALVATE VGEEAAPDDV GVGEVPWTPV MRTFGDRVTS AAFAQWVTLG APAGLGLEVL
AGGLGAVLDA HDMLRARTVP GEPRLIVGER GSVDASALVS RVEAAAGEVS EVAERAAREA
AGRLDPASGV MVQAVWVDAG VGRVGQLVVV VHHLVVDGVS WRVLVPDLQG ACEAVAAGHV
PVLDAVGTSF RRWSGVLAAE AAGERRVAEL GDWLALLGEG QFPLGQRELD PALDTVRTLR
RQSWVVPPER AAALVGRTPT AFHCGVDDVL LAALVGAVAH WRPDAASGVL VDIEGHGREP
LGGVELSRTV GWFTSAHPVR LDATGMELSQ VLAGGPVAGR LLKAVKEQAR AVPGDGLGYE
LLRHLNPETG PVLKAAPTPQ IGFNYMGRFT SGGTTGTGTS TDTGTGQAEA APWQMAGPTA
IGGSVDPDMP VTHLLEVGAA VRDTPDGPEL TLTLSWPGQL LEDADAERLG RTWAEMLGGL
AVHTAASGSG GHTPSDFPLL DGVLTQDGVA ELEETVTGLA DIWPLSPLQE GMLFHATYDD
AAPDVYEGQR ALALDGPLDA ARLRAAWETV VRRHPILRAS FHRLASGDAV QVVARNVELP
WREADLSDRT EAEALVEAER LAAVEAAERI DVTRAPLLRL LLIRLGAERH HLVITSHHIT
MDGWSLPVLV GDLSAAYEAH AAGRDERDLP AVTSYREYLA WLNRQDKEVA RDAWRAELAG
LDEPTLVVPA ETVRVPVVPD RIRFTFSRDL SRGIAGLARG HGLTVNTVVQ GAWALLLARL
ARRTDVVFGA TVAGRPPDLP GAESAIGLFI NTLPVRVSLT PGQPVVEMLA ELQRRQVSLM
AHQHMGLSEI HQLAGQGGVF DTLVVYENYP RPPEGGSAPG SLRIRPAGHP QDMGHYPLTF
VVSPGERMQG DFVFRPDVYD RARAEGMVAS LVRILEQLVA DPSAPLGRVT VVDTAVRDRV
VERWNRTREP ATGAVPLPEL FGRQVVRSPA AVALVSGEQA LSYRELADGA GRLARYLTRL
GVGPEQRVAV VGERSATTVA ALLAVSLAGG VFVPVDAGYP AERIAYMLAD AAPAVVVCTR
RSRDAVPAGF PGRVVVTDAP GVADEVRRCA GGPVRDGERS APLSAANAAY VIYTSGSTGT
PKGVVVTHAG LGNLARAQID RFGVRADSRV LQFASLSFDA AVSELCMALL SGAALVVAGP
DGLPPRVSLE EAVRRARASH VTVPPSALAA EEELPDTLET VVVAGEACPP GLVDRWSAGR
RMVNAYGPTE TTVCAAMSEP LSPGADVVPL GRPMANVRVF VLDEFLLPVP PGVAGELYVA
GAGLARGYLE RAGLSAGRFV ACPFGAGERM YRTGDVVRWT GGDGGGDEGG GGGELLFVGR
ADDQVKVRGH RIEPGEIEAV LTGHPDVTHA VVVAHRDRPD DNRLVAYVVT DTARRTDAEE
AASGEGAVDA ERAAGTVAAL PAYLRERLPE HMVPAVFVPL GRLPLTPNGK VDRAALPAPD
FAGRVTGGGP RTETERVLCG LFAEVLRLER VGVADSFFEL GGDSIMSMQL ASRARRAGLL
LTSRQVFEEK TPERLAEVAE VAEPETAVVD IGVGEVSWTP VMRALGERAA RPGFAQWVVA
GAPPGLGLET LAAGLGTVLD THDMLRARTV PGEPRLIVGE RGSVAAASAV VRVDAADAAD
DALDEVAGRA ARAAVERLDP AAGVMAQAVW VDAGAGRTGR VVLVAHHLVV DGVSWRILLP
DLRAACEAAA VGRAPEPEPV GTSFRRWAEL LAAQAADERR VAELDDWTAL LGEPRPPLGR
RELDPEVDTV RTLRHRTWVV PPEQAATLVG RTPGAFHCGV DDVLLATLAG AVAHWRPGLA
AGLLVDVEGH GREPLEGADL LRTVGWFTSV RPVRLEVTGV DLDAARRGGR AAGSLVKAVK
EQARAVPGDG LGHELLRHLN PVTGPVLAAA PTPQIGFNYL GRFAAGGEAG TGPVGPWQPA
GESAIGGSVD PGMPATHAVD AGAVVRDTAD GPELTITLSW TSRLLEEADA TRLGETWLTM
LDGLAAHTAD PTAGGHTPSD FHLLNLAQHQ IEELEAGFAG EQP
//
