ID A0A0N1PC46_LEPSE Unreviewed; 1060 AA.
AC A0A0N1PC46;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=mRNA (guanine-N(7))-methyltransferase {ECO:0000256|ARBA:ARBA00011926};
DE EC=2.1.1.56 {ECO:0000256|ARBA:ARBA00011926};
GN ORFNames=ABL78_5933 {ECO:0000313|EMBL:KPI84998.1};
OS Leptomonas seymouri.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leptomonas.
OX NCBI_TaxID=5684 {ECO:0000313|EMBL:KPI84998.1, ECO:0000313|Proteomes:UP000038009};
RN [1] {ECO:0000313|EMBL:KPI84998.1, ECO:0000313|Proteomes:UP000038009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 30220 {ECO:0000313|EMBL:KPI84998.1,
RC ECO:0000313|Proteomes:UP000038009};
RX PubMed=26317207;
RA Kraeva N., Butenko A., Hlavacova J., Kostygov A., Myskova J., Grybchuk D.,
RA Lestinova T., Votypka J., Volf P., Opperdoes F., Flegontov P., Lukes J.,
RA Yurchenko V.;
RT "Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by
RT Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania
RT donovani.";
RL PLoS Pathog. 11:E1005127-E1005127(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA +
CC S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-
CC triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA-
CC COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461,
CC ChEBI:CHEBI:167617; EC=2.1.1.56;
CC Evidence={ECO:0000256|ARBA:ARBA00024288};
CC -!- SIMILARITY: In the N-terminal section; belongs to the dsDNA virus mRNA
CC guanylyltransferase family. {ECO:0000256|ARBA:ARBA00008556}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPI84998.1}.
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DR EMBL; LJSK01000215; KPI84998.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0N1PC46; -.
DR EnsemblProtists; KPI84998; KPI84998; ABL78_5933.
DR VEuPathDB; TriTrypDB:Lsey_0215_0010; -.
DR OMA; ETGHGDC; -.
DR OrthoDB; 167976at2759; -.
DR Proteomes; UP000038009; Unassembled WGS sequence.
DR GO; GO:0004482; F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR004971; mRNA_G-N7_MeTrfase_dom.
DR InterPro; IPR039753; RG7MT1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12189:SF4; MRNA (GUANINE-N(7))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR12189; MRNA GUANINE-7- METHYLTRANSFERASE; 1.
DR Pfam; PF03291; mRNA_G-N7_MeTrfase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 811..1018
FT /note="MRNA cap 0 methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF03291"
SQ SEQUENCE 1060 AA; 118058 MW; 30C6508262EDB260 CRC64;
MLNVNAKAFV PPGVVEGVPS SFIPNAWYRY RLNMPIENRM RSVHEERYLN NISSLLSLKY
LYAERGGLDD EALQYFLNKQ FLLSNNSVSK ENAESASSGF LLSRSILTLY TDLTAVTNLN
DSKGLLVHLD LPTEGSGDVA VFLASAWENF YAAVTENSRV SAIGYSRSVV GLNGSETGGT
YRHYFFLACN TPELLEPFLS GVMANCPVRV HCILPVKSIV PYICCPHLQF GVAITFGKDA
KRSLTKNRKA DLDVEIKYKA DDFHEAWKEI KRAQGILEEF LSGDTKWDRT LILHLHVDVI
STTKSVVVSI CDVIREIGFF CDDAGAGIIG VSFSSDFDDH FVEQLVHRNK NGPFYNCGTL
DEMLKKDLSL DTRNIYFGGF PSHVKTVLPT TAYDDAVSAL ELWQRFEKQM PATELLTRMT
FADAADSFAR APYIVRRRAV GANVLIGTDA RGSIFGVDVA HNSVFGLPMC FGGAIAPVIN
CVFKGTVSPS YRSSLDYRVI VDDLLVFEGK ETKGIPYTER LRMLSGIGIE DETTWPHANA
AHIVILKAEC VPLEHTKELV ETPVFDHNNL GIVFSPSVQP DNEAILPIFK WVPPTSLTAR
FAVECIESSE DVSEGFSRAF LGVYSSNVQR KIIIPYEGEY ADYHTEAYPN LKAGSLVECL
LRRADDGAHW WDVLKALDST ESIMAHSFDE VDDLVHSPGV TQDEMLWLLK APSYQCERCR
RVNDVGRRNN RHNAYWCKSC WIETGHGDCM YCGRSFALGM LDGRSNLFYC EDCWNAFSST
NTEAEVGYHV PPPPNATFTQ QVTTRCVSLL IDLMNSKTPS NDVLDLCCGG SVLRKWMRNR
TSRYVGIDLK RSVIDSLTSI IHNAKERVPG GQYDAVCGDV FSDDFWVSTI VKIHPRQFHV
ISCFAGLHHA FENEKKARHF LGSVANALVP GGTFLGAFID ASVLFSRGKK YSNGIMKAGW
SENSVPRIGH TFQFNTDNGP LRDVNVIPID FLIAVAAEYG LHAVSEAYMT FRELIEKDAS
WTKVPSADEK EYLCALRTFA FKKESSELAP SSPGKRSDSN
//
