ID   A0A0N5CB34_STREA        Unreviewed;       943 AA.
AC   A0A0N5CB34;
DT   09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT   09-DEC-2015, sequence version 1.
DT   22-FEB-2023, entry version 21.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Strongyloides papillosus (Intestinal threadworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Tylenchina; Panagrolaimomorpha; Strongyloidoidea; Strongyloididae;
OC   Strongyloides.
OX   NCBI_TaxID=174720 {ECO:0000313|Proteomes:UP000046392, ECO:0000313|WBParaSite:SPAL_0001509800.1};
RN   [1] {ECO:0000313|WBParaSite:SPAL_0001509800.1}
RP   IDENTIFICATION.
RG   WormBaseParasite;
RL   Submitted (FEB-2017) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0N5CB34; -.
DR   STRING; 174720.A0A0N5CB34; -.
DR   WBParaSite; SPAL_0001509800.1; SPAL_0001509800.1; SPAL_0001509800.
DR   Proteomes; UP000046392; Unplaced.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF2; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944}.
FT   DOMAIN          182..502
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          567..933
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
SQ   SEQUENCE   943 AA;  109751 MW;  78C8393FCAE85FF1 CRC64;
     MTPPKNVLLN TSKSFSYNTT SSDEKSMCFD GTFTNVSERE DILPKHLDGF EHKNNINTSN
     IITNNHSSLE NFTKDIKEHS MTNHYPKEYI EDDSNKNIMY KNCDSTNSLS LESYINTYGW
     IAKGSKKPID HRLPFQRPPL IHCVKQLPFA LRYLGYWIKN YKKRETLFIN GFEPYFHHPY
     YGVPLGGIGS GSIGRDFRGG FCKFSLRPGI VEQKVKFIKA NQFILSIKKD GITIYQKVLS
     AVMTMNKKKK KLKKSIKKRN DYLSSWVFNF PEDDIEYRGL YPRSWTKYNI KECNVIVTCK
     QTSPVIPHDY KNSSLPTSIF EFEVENNSND DLDISITFTW RNGTGNMKWN KEGICETKSL
     DINDYDNNIK SVILKNSICG MNCIYGLSGI QKENQNISIC EKFNPSGDGY NLWNELKNNG
     KLEKIADNNF NDNEIGVGIC ISENIKSSTK TSFTFNLLWH MPLIYFGNKK RLMKRRYTRY
     FGCDDEAIKD LISYSFLNYP NWTKEIEKWQ NPILTNKDYP DWFKSALINE LYYLTDGGSI
     WIEYDDDWKK NEPSISEYTE NVLKEYGRFG YIESWDYKMV NTYDVHFYAS FAIATHFPYL
     EHSIVSDFGD QVNRSEDKLT KYYMENVKAP IKTPQRLPHD LGNPAIEPYI QSNCYVMHDT
     AHWKDLNLKY VLTAYRNYYT LRNNDIEFLR FVYPKVKSLI EVGITEWDTN NDGMIENFGA
     ADQTYDAWKM VGCSSYCGSL WLSALRVSIE MAKDMGDTET EKRYRDILEK AKNVFNNKLW
     NGKYYNFDES RLSSRTIMAD QLAGYWFLYS ISKDLANSIL PFDRVYSSLI TVYNFNCKKF
     NNGNMGVVNG MKPNGKVDTD HIQADEMWTG ITYAVASSLI QVGEIEKGFE VAYGCYNTCY
     NNTGLQYQTP EALYSKNFYR AIGYMRPLSI WAMNWELSKN KKI
//