ID A0A0P0NKU6_9SPHI Unreviewed; 666 AA.
AC A0A0P0NKU6;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 13-NOV-2019, entry version 21.
DE RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN ORFNames=AQ505_24680 {ECO:0000313|EMBL:ALL08385.1};
OS Pedobacter sp. PACM 27299.
OC Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Pedobacter; unclassified Pedobacter.
OX NCBI_TaxID=1727164 {ECO:0000313|EMBL:ALL08385.1, ECO:0000313|Proteomes:UP000062859};
RN [1] {ECO:0000313|EMBL:ALL08385.1, ECO:0000313|Proteomes:UP000062859}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PAMC 27299 {ECO:0000313|EMBL:ALL08385.1,
RC ECO:0000313|Proteomes:UP000062859};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC molecules used as primers for DNA polymerase during DNA
CC replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC ECO:0000256|SAAS:SAAS00709340}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|SAAS:SAAS00709317};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
CC -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC domain that contains the primase activity, and a C-terminal DnaB-
CC binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC -!- SIMILARITY: Belongs to the DnaG primase family.
CC {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
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DR EMBL; CP012996; ALL08385.1; -; Genomic_DNA.
DR RefSeq; WP_062550613.1; NZ_CP012996.1.
DR EnsemblBacteria; ALL08385; ALL08385; AQ505_24680.
DR KEGG; pep:AQ505_24680; -.
DR KO; K02316; -.
DR OrthoDB; 1071997at2; -.
DR Proteomes; UP000062859; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR Gene3D; 3.90.580.10; -; 1.
DR Gene3D; 3.90.980.10; -; 1.
DR HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR InterPro; IPR013264; DNA_primase_core_N.
DR InterPro; IPR037068; DNA_primase_core_N_sf.
DR InterPro; IPR019475; DNA_primase_DnaB-bd.
DR InterPro; IPR006295; DNA_primase_DnaG.
DR InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR InterPro; IPR030846; DnaG_bac.
DR InterPro; IPR034151; TOPRIM_DnaG_bac.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR002694; Znf_CHC2.
DR Pfam; PF10410; DnaB_bind; 1.
DR Pfam; PF13662; Toprim_4; 1.
DR Pfam; PF08275; Toprim_N; 1.
DR Pfam; PF01807; zf-CHC2; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SMART; SM00400; ZnF_CHCC; 1.
DR TIGRFAMs; TIGR01391; dnaG; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000062859};
KW DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993445};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709369};
KW DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709327};
KW Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709338};
KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709339};
KW Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709304};
KW Reference proteome {ECO:0000313|Proteomes:UP000062859};
KW Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709341};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993442};
KW Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709301}.
FT DOMAIN 260 341 Toprim. {ECO:0000259|PROSITE:PS50880}.
FT ZN_FING 37 61 CHC2-type. {ECO:0000256|HAMAP-Rule:
FT MF_00974}.
FT REGION 439 476 Disordered. {ECO:0000256|SAM:MobiDB-
FT lite}.
FT COMPBIAS 439 466 Polar. {ECO:0000256|SAM:MobiDB-lite}.
SQ SEQUENCE 666 AA; 74575 MW; 3FC029D9B51D3191 CRC64;
MINQDTISKI METVRIEEVV GDFVSLKKRG TSLIGNCPFH NEKTPSFHVS VAKGIYKCFG
CGKGGDSVHF IMDHEKYSYP EALKFLAQKY HIEVEETVQS PQNIEAQNAR ESLYIVSEFA
AGFFANELWT GDQGRAIGLS YFKERGFRED IVKKFQLGYS PDVWDALIQH AVSAGHKEEY
LEKTGLAIRN DKGKLYDRFR GRVMFPIHNF TGRIIGFGGR TLKTDKNVPK YVNSPESDIY
HKSNVLYGLF HAKKAIRDTD NCYLVEGYAD VLAAHQAGIE NVVASSGTSL TTEQIRLIGR
FTENVTILYD GDAAGIKASL RGLDMILEEG LNVKVVSFPD GHDPDSYMHH VGSGAFKKYI
EEHRKDFILY KANILLKEAG SDPIKRAGII RDIVESIAKI PDDIKASIFI KECSSLLQVE
ERILLSELNK IRMAKFRKSS AGQSGTGNSG VGSSGFQQQN QYQPYPDGPP DNLFEVPGAA
GEAAEAAAAE ESDLLQEQEV VRLLLAYGHE LVSWDKIDNM YIGSFIMQNL ADVSFTDPTC
KIIIDTYNEE IENGHLPVAS QFINHENRNI VELAITLSTS PYSLSENWYN KHNIYVRDES
VNLKATVLGG IFHLKKGKVA RMLSDISKEI KAENDPVNLE ILMQRFMFMK SVEREISKFL
GTVILK
//
