ID A0A0P0S8I9_9PSEU Unreviewed; 1185 AA.
AC A0A0P0S8I9;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=AD017_02705 {ECO:0000313|EMBL:ALL80395.1};
OS Pseudonocardia sp. EC080619-01.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Pseudonocardia.
OX NCBI_TaxID=1096856 {ECO:0000313|EMBL:ALL80395.1, ECO:0000313|Proteomes:UP000066228};
RN [1] {ECO:0000313|EMBL:ALL80395.1, ECO:0000313|Proteomes:UP000066228}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EC080619-01 {ECO:0000313|EMBL:ALL80395.1,
RC ECO:0000313|Proteomes:UP000066228};
RX PubMed=26535611; DOI=10.1021/jacs.5b09794;
RA Van Arnam E.B., Sit C.S.;
RT "A Rebeccamycin Analog Provides Plasmid-Encoded Niche Defense.";
RL J. Am. Chem. Soc. 137:14272-14274(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP012184; ALL80395.1; -; Genomic_DNA.
DR RefSeq; WP_010227655.1; NZ_CP012184.1.
DR AlphaFoldDB; A0A0P0S8I9; -.
DR STRING; 1096856.AD017_02705; -.
DR KEGG; pecq:AD017_02705; -.
DR PATRIC; fig|1096856.3.peg.573; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000066228; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000066228};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..77
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1185 AA; 129920 MW; 6C72A26F1DDBF65E CRC64;
MASSGAGSFV HLHTHTEFSM LDGAARLDDL FSEAVKNDMS AVAMTDHGNV YGAFEFWRKA
KAAGVKPIIG MEGYLAPGSR HERKRVALGG SNPGAADKNP DLMYTHMTLL AENTEGMHNL
FRLSSLASLE GYYYKPRMDR ELLEQYGTGL IGTTGCPSGE VARLLQQDDF EGACQAASDY
RDIFGKDNFF CELMDHGIEI EKRVQEDLFA LKKRLDLPGL ATNDLHYTYP DDTKAHEVLL
CVQTGKTLAD PNRFKFDAQD FYLKSATEMR ELWDPIHPEA CDNTLWVAER CDIDFTTGQD
LQPKAPVPEG ETEESWLIKE VERGLHDRFP NGITEQYRTQ ANYEVGVIIK MGYPGYFLVT
ADLIRHAKSV GIRCGPGRGS AAGSLVAYVL GITDLDPIRH KLIFERFLNP DRVSMPDIDM
DFDERRRGEM IRYVTEKYGE DRIAQIITYS TIKAKAAIKD SARVLFGQPG YSVADRISKA
MPPAVMGKDI PLSGIFDPSH KRYSEATEVR ALYEADPQVK EIVDTARGLE GLKRQWGVHA
AGVIMSSTPL IDVIPIQRRE SDGAVITQFD MGVCETIGLL KMDFLGLRNL TILDDALENI
TLNGKPEVDL DHVELDDGPT YELLSRGDTL GVFQLDGGPM RDLLRRMAPS EFEHISAVGA
LYRPGPMGAN AHNDYADRKN GRQEITPIHP ELAEPLKDVL GETFGLIVYQ EQVMSIAQVL
AGYSLGQADL LRRAMGKKKK EILDKEYVGF AQGMKDNGYS ADAVKTLWDI LLPFSDYAFN
RAHSAAYGVV SYWTAYLKAN YPAEYMAGVL TSVRDDKDKA AVYLAECRRM GVTVLPPDVN
DSVRNFAPVG NDIRFGLGGI RNVGHNVVDA IVAARREKGN FVDFSDFLRK VDAVVCNKKV
VESLIKAGAF DSLGHSRKGL LLVHADAIDA VMSTKKAASL GQFDLFGSMD GSGGDDGDEL
GGIFDVKVPE EEWDSKHKLA VEREMLGLYV SGHPLQGLEQ ALAAKADTSI ADIVEGSVAE
GGQVTVGGIL ANVNRRVNKN GEPWASAQIE DLAGGIEVLF FPRAYQVVGV DVAEDAIVLV
KARVNKRDDR ISLIANDLAV PELAAAGEGG LPIRVSLRTD QCTPERVSKL KEVLTHHPGT
SEVHLSLVYG SKITALRLDD SLKVMHTSAL MGDLKALLGP GCLGG
//
