ID A0A0P7HZV2_9EURY Unreviewed; 178 AA.
AC A0A0P7HZV2;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Crossover junction endodeoxyribonuclease Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_01490};
DE AltName: Full=Holliday junction resolvase Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN ORFNames=SY89_00644 {ECO:0000313|EMBL:KPN29924.1};
OS Halolamina pelagica.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Halorubraceae.
OX NCBI_TaxID=699431 {ECO:0000313|EMBL:KPN29924.1, ECO:0000313|Proteomes:UP000050535};
RN [1] {ECO:0000313|Proteomes:UP000050535}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CDK2 {ECO:0000313|Proteomes:UP000050535};
RA Hoang H.T., Killian M.L., Madson D.M., Arruda P.H.E., Sun D.,
RA Schwartz K.J., Yoon K.;
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are
CC subsequently ligated to produce recombinant products.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10;
CC Evidence={ECO:0000256|ARBA:ARBA00029354, ECO:0000256|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPN29924.1}.
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DR EMBL; LGUC01000001; KPN29924.1; -; Genomic_DNA.
DR RefSeq; WP_054583072.1; NZ_LGUC01000001.1.
DR AlphaFoldDB; A0A0P7HZV2; -.
DR STRING; 699431.SY89_00644; -.
DR PATRIC; fig|699431.3.peg.664; -.
DR OrthoDB; 34330at2157; -.
DR Proteomes; UP000050535; Unassembled WGS sequence.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; Holliday_junction_resolvase; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR NCBIfam; NF040854; Hol_resolv_Hjc; 1.
DR PANTHER; PTHR39651; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR PANTHER; PTHR39651:SF1; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR Pfam; PF01870; Hjc; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01490};
KW Reference proteome {ECO:0000313|Proteomes:UP000050535}.
FT ACT_SITE 31
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 11
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 41
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 54
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT SITE 56
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
SQ SEQUENCE 178 AA; 19161 MW; 3F6A4284CFA6E4B5 CRC64;
MPTNRKGDRR ERELVNRLDD AGFAVMRAPA SGGATQRELP DVLAGNGESF YAIEAKSSAG
DPIYLTGEEV QALVYFAQNF GAKPRIGVRF DREDWYFFHP GDCYTTDGGN YRVKKETALA
DGTDMAELVG DTEKVTLAEA TERGTATTGD DADDGVRKLL QAVADGTLSV EEAADALA
//
