ID A0A0P7ZEI6_9EURY Unreviewed; 412 AA.
AC A0A0P7ZEI6;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01893};
DE AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
DE Short=Psi54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
GN Name=pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
GN ORFNames=MPEBLZ_03514 {ECO:0000313|EMBL:KPQ41935.1};
OS Candidatus Methanoperedens sp. BLZ1.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Candidatus Methanoperedenaceae; Methanoperedens.
OX NCBI_TaxID=1719120 {ECO:0000313|EMBL:KPQ41935.1, ECO:0000313|Proteomes:UP000050360};
RN [1] {ECO:0000313|EMBL:KPQ41935.1, ECO:0000313|Proteomes:UP000050360}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Arshad A., Speth D.R., De Graaf R.M., Op Den Camp H.J., Jetten M.S.,
RA Welte C.U.;
RT "A metagenomics-based metabolic model of nitrate-dependent anaerobic
RT oxidation of methane by Methanoperedens-like archaea.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC Rule:MF_01893}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01893};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01893};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC {ECO:0000256|ARBA:ARBA00009652, ECO:0000256|HAMAP-Rule:MF_01893}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPQ41935.1}.
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DR EMBL; LKCM01000282; KPQ41935.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0P7ZEI6; -.
DR PATRIC; fig|1719120.3.peg.3813; -.
DR Proteomes; UP000050360; Unassembled WGS sequence.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.2510; -; 1.
DR Gene3D; 3.30.70.3190; -; 1.
DR HAMAP; MF_01893; Pus10_arch; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR005912; Pus10.
DR InterPro; IPR039894; Pus10-like.
DR InterPro; IPR048741; Pus10-like_C.
DR InterPro; IPR004114; THUMP_dom.
DR NCBIfam; TIGR01213; pseudo_Pus10arc; 1.
DR PANTHER; PTHR21568:SF0; TRNA PSEUDOURIDINE SYNTHASE PUS10; 1.
DR PANTHER; PTHR21568; UNCHARACTERIZED; 1.
DR Pfam; PF21238; Pus10_C; 1.
DR Pfam; PF02926; THUMP; 1.
DR SMART; SM00981; THUMP; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS51165; THUMP; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01893};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01893, ECO:0000256|PROSITE-
KW ProRule:PRU00529};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_01893}.
FT DOMAIN 55..172
FT /note="THUMP"
FT /evidence="ECO:0000259|PROSITE:PS51165"
FT ACT_SITE 241
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
FT BINDING 307
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
FT BINDING 377
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
SQ SEQUENCE 412 AA; 45968 MW; 8A241F3DBCC64041 CRC64;
MTILDTARKI VHEGPICDHC LGRQFAKLST GLSNDMRGAA LKLVLAMHAG AEKDKELQDE
LARSRGEIKC WVCNNLFLKI DPWVTKSIEV LSDYEYNNFL VGTKVTGLLA ENEEILWAES
GTTFAEPLKT ELNREVGKRI EKLTGKRANL KKPQVVVLLD LENDTVDVEI NSLYIYGRYR
KLIRGIPQTR WPCRECGGRG CERCDNTGKM YQESVDELIK PHLMSAARCA DTAFHGAGRE
DIDALMLGDG RPFVVEAKRP HIRSLDLEAL EAGINRGAQG KIEVLGLKFV ESETVEQLKS
MKADKVYRLK VTHNTTEEKL KSSINVISQT LIKQKTPTRV LHRRADLERV RKVHSVELES
YNENTAVLVI NCEGGLYVKE LVSGDGGRTI PSVASLTGSE AKVIELEVIK VG
//
