ID A0A0P7ZM23_9EURY Unreviewed; 860 AA.
AC A0A0P7ZM23;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=MPEBLZ_00097 {ECO:0000313|EMBL:KPQ45300.1};
OS Candidatus Methanoperedens sp. BLZ1.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Candidatus Methanoperedenaceae; Methanoperedens.
OX NCBI_TaxID=1719120 {ECO:0000313|EMBL:KPQ45300.1, ECO:0000313|Proteomes:UP000050360};
RN [1] {ECO:0000313|EMBL:KPQ45300.1, ECO:0000313|Proteomes:UP000050360}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Arshad A., Speth D.R., De Graaf R.M., Op Den Camp H.J., Jetten M.S.,
RA Welte C.U.;
RT "A metagenomics-based metabolic model of nitrate-dependent anaerobic
RT oxidation of methane by Methanoperedens-like archaea.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU000442};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPQ45300.1}.
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DR EMBL; LKCM01000014; KPQ45300.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0P7ZM23; -.
DR PATRIC; fig|1719120.3.peg.107; -.
DR Proteomes; UP000050360; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd05160; DEDDy_DNA_polB_exo; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00592; pol2; 1.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442}.
FT DOMAIN 57..320
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 389..831
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
SQ SEQUENCE 860 AA; 98049 MW; 51A5C343172658E6 CRC64;
MKFQILNADY TYSDDLPVIR LYGRDESGNS ICCHVPGFEP YFYAKAKPEL STILLEKFKE
HIKRIEQVKR FEPVGYFKNK TSMLKIILYD PKSVPIIRDD IRKMVDEIYE TDILFRNRYM
VDKGLGGMGW AKAEPEKDTI SNEVISDLKI TSGNVEPVEI LKNAPLRHLA FDIECLPLNG
AMPVPETSPI VLISLAFAPD FEGKKTLVLV GKDAKADDET ESCGSEEAML KRFFEIIKKY
DPDILVGYNS NSFDIPYIVD RMRTLNRKGA RIEPIAGRDG RDLYYKKIGN VTRISVMGRI
AVDVLPLLRR EFSLKQYTLR NTAKELLGSE KLEIPFLEME DYWKDNGEKL SKFIEYSRRD
SELALLFLLK LQLIDKYIAL ARVSGTLLQD ILDGGQTQMV ESLMLREYMK NDRVLPARPT
GEMSDERYDE GEELAGAEVL PPKKGLLENI VILDYKSLYP TIMMAHNLCY TTEVVGERPA
DVIVAPTGGV FVSSKVVKGI VPSILEDLLN RRQETRAKMK TANEEEKRVL DATQLALKIL
LNSFYGYSGY TRARLYSLTL ASAVTSFGRE NILRTKDLIE KDIREIILKD GKAFKKDENI
SGKRIGLSVV YGDTDSVFVH LADKEINFDE AQLVGNTIAN TVTDSLVKPM ELVFDSFARR
AIFLAKKRYA LLIQEKTAKG LKEKIKVKGM ETVRRDWCEL TTKTIEKVLE LVLKEGKVDD
AVLLVKNTIN SIKTIDSTSP LFDDLILTRQ YTKKIESYRN RQPHITVIEK LQKRGIISHV
GDRIPFVIVA GNAIFVDRAE DPEYAQKKNL PIDVDYYINK QILPPVERIL SDFGVTREML
RGMGERKVQT ETKQKQLFEF
//
