ID A0A0Q0XD37_9PSED Unreviewed; 548 AA.
AC A0A0Q0XD37;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=AQS70_05365 {ECO:0000313|EMBL:KQB55549.1};
OS Pseudomonas endophytica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1563157 {ECO:0000313|EMBL:KQB55549.1, ECO:0000313|Proteomes:UP000050342};
RN [1] {ECO:0000313|EMBL:KQB55549.1, ECO:0000313|Proteomes:UP000050342}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BSTT44 {ECO:0000313|EMBL:KQB55549.1,
RC ECO:0000313|Proteomes:UP000050342};
RA Von Neubeck M., Huptas C., Wenning M., Scherer S.;
RT "Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov.,
RT isolated from raw cows milk.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQB55549.1}.
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DR EMBL; LLWH01000002; KQB55549.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0Q0XD37; -.
DR STRING; 1563157.AQS70_05365; -.
DR Proteomes; UP000050342; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Endonuclease {ECO:0000313|EMBL:KQB55549.1};
KW Hydrolase {ECO:0000313|EMBL:KQB55549.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:KQB55549.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..217
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 380..497
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
SQ SEQUENCE 548 AA; 61561 MW; 9ABA1D02DD4D843F CRC64;
MLSVAQLPDR GEIRVLDPAA GDGALLEALL KRMPVDTLSR VRVFGYDTDP SAVVRASARL
QNAFPDVSIL IEEKDFLDHV LSLSADSDLF SDTRSTLMPF DLVIANPPYV RTQIMGTEQA
QKLAQSFALT GRVDLYYPFL LGISRVLSEG GVTGIITSNR FMTTKSGQAV RRALLSKFRI
MHVWDLGDTK LFDAAVLPAV LLAKGTKQIV DSDVEDIVFS TIYESKENAV DHVSNVMEAL
TMSDNTVVSI SDGRNFLVRH GLLDNGESAD GIWRISTASS DQWLATVHKH TWNTFRRVGK
IRVGVKSTAD KVFVRSDWSA LPGGRPELLR PLITRYCARQ FKACIPSKTK HIKEIMYPHE
STSKGRKAVD LSLYPIAARY LELHRTTLEA RSYLIKAGRA WYELWVPQDP AAWSSPKIVF
PDISEKPTFW MDTEGGIVNG ECYWLQCERE GENDLLWLAL AVANSTFIEF FYDHCFNNKL
YAGRRRFITQ YVEQFPLPDP ARKESQIIID LAKAIHSELP SNAADQKISE LNALIWNVFG
LNVEEVPR
//
