ID A0A0Q3F078_BRADI Unreviewed; 276 AA.
AC A0A0Q3F078;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=General transcription and DNA repair factor IIH subunit TFB4 {ECO:0000256|RuleBase:RU368090};
DE AltName: Full=RNA polymerase II transcription factor B subunit 4 {ECO:0000256|RuleBase:RU368090};
GN Name=100830439 {ECO:0000313|EnsemblPlants:KQJ93071};
GN ORFNames=BRADI_3g02580v3 {ECO:0000313|EMBL:KQJ93071.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EMBL:KQJ93071.1};
RN [1] {ECO:0000313|EMBL:KQJ93071.1, ECO:0000313|EnsemblPlants:KQJ93071}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQJ93071.1,
RC ECO:0000313|EnsemblPlants:KQJ93071};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:KQJ93071.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQJ93071.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQJ93071}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:KQJ93071};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000256|RuleBase:RU368090}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of XPB,
CC XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5,
CC which is active in NER. The core complex associates with the 3-subunit
CC CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD
CC and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH)
CC active in transcription. {ECO:0000256|RuleBase:RU368090}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU368090}.
CC -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000256|ARBA:ARBA00005273,
CC ECO:0000256|RuleBase:RU368090}.
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DR EMBL; CM000882; KQJ93071.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0Q3F078; -.
DR EnsemblPlants; KQJ93071; KQJ93071; BRADI_3g02580v3.
DR Gramene; KQJ93071; KQJ93071; BRADI_3g02580v3.
DR Proteomes; UP000008810; Chromosome 3.
DR ExpressionAtlas; A0A0Q3F078; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12831:SF0; GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3; 1.
DR PANTHER; PTHR12831; TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED; 1.
DR Pfam; PF03850; Tfb4; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU368090};
KW DNA repair {ECO:0000256|RuleBase:RU368090};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|RuleBase:RU368090};
KW Nucleus {ECO:0000256|RuleBase:RU368090};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU368090};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU368090}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|RuleBase:RU368090};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|RuleBase:RU368090}.
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 276 AA; 29577 MW; 6E25D5AD1BB7DC2A CRC64;
MTSAHSKLYS DDVSLVVVIV DTNPFFWAGA ALPFADFFGH LIHFVNSLLL LNHLNQVVVI
AAGVSSCAYV FDSGNAAASG SPDVAATFAK ASRKVEEFVK QDARDTAGNG TVAGGGAASL
FSGALSLALC YIQRVFRSGT RHPQPRILCL QGSPDGPEQY VAVMNSIFSA QRSMVPIDSC
IVGTQDSAFL QQASYITGGV YLKPQELSGL FQYLAAVFAS DLHSRAFLRL PKTLGVDFRA
SCFCHKKTID MGYVCSVCLS IFCKYHKKCS TCGFCC
//
