ID A0A0Q4G5Q8_9SPHN Unreviewed; 1156 AA.
AC A0A0Q4G5Q8;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ASE72_00655 {ECO:0000313|EMBL:KQM73219.1};
OS Sphingomonas sp. Leaf20.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1735685 {ECO:0000313|EMBL:KQM73219.1, ECO:0000313|Proteomes:UP000051642};
RN [1] {ECO:0000313|Proteomes:UP000051642}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf20 {ECO:0000313|Proteomes:UP000051642};
RA Garrido-Oter R., Mueller D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000051642}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf20 {ECO:0000313|Proteomes:UP000051642};
RA Vorholt J.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQM73219.1}.
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DR EMBL; LMKN01000001; KQM73219.1; -; Genomic_DNA.
DR RefSeq; WP_056015507.1; NZ_LMKN01000001.1.
DR AlphaFoldDB; A0A0Q4G5Q8; -.
DR STRING; 1735685.ASE72_00655; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000051642; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000051642}.
FT DOMAIN 625..786
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 807..961
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1156 AA; 126642 MW; 71474353DD88855A CRC64;
MPDLNTILSA TTPLTLSGVP SGFQPSLLAD LARAAKTRAV FIAPDDAAMR AIASTAAFFA
PDLEILSFPA WDCLPYDRAS PTLRVMAERV AALQRLQGKP KGPQLLLTTA NAATQRVLTP
FRIRQLVARL APGERIGRDK LATLLQANGY VRTDTVHDQG EYAVRGGIVD LYPSGEDHAL
RLDFFGDEIE SVRTFDAADQ RTTGRIDGFV LLPASEALLD EDSIKRFRTR YRDAFGATAT
GDPMYQAISE GRRIAGMEHW LPLFEEKMAT LFDHLGADDV LVRDSGVAAA AESRLESIAD
YYENRKRAEA AQPGSYRPMP AKALYLDAQD WSGATEAFAA HITSPFHEPE SATVLDFAVD
GPRDFGPERA AQANIYEAVA DHVEKLRKQG RKPILASYSI GARERLGSLL ADHGMKGGKH
AETWQEALGV ADTAKSFGVA LIVLPLDHGF TAPGVAVLTE QDMLGDRLVH RKKRKKSADA
FLAELATLTP GDLVVHTEHG IGRYEGLTSI PVGQSPHDCV ALSYAGGDKL YVPVENIDVL
SRYGSSEEGA SLDKLGGEGW QRRKSKMKER IREIAGELIA TAAERALHPG DVLEPDSSGY
PSFVDRFPYE ETEDQDRAIE EVLADLSAGK PMDRLVVGDV GFGKTEVALR AAFVAAMGGK
QVAIVCPTTL LARQHYNNFV SRFEGFPMNM GRLSRLVTAS EAKATKEGLA NGTIDVVIGT
HALLAKNIDF KRLGLVVVDE EQRFGVTHKE RLKALRADVH MLTLTATPIP RTLQMAMSGI
RELSVIQTPP VDRLAVRTYI MPFDPVVLRE ALLREHYRGG QSFVVTPRVA DLPEIEEFLR
EQVPEIRFVV AHGQMSPTEV EERMSAFYDK KFEVLVSTTI IESGIDIASA NTMIVHRADR
FGLAQLYQLR GRVGRSKTRA YAYLVTPPER QMTVTAEKRL KVLSDLDSLG AGFQLASHDL
DIRGAGNMLG DEQTGHIKEV GFELYQAMLE EAIMDAKSGG LASARPKDFS PQITVDAPIL
IPEEYVPDLD LRMGLYRRLN ELDEGQEIEA FAAEMIDRFG PLPDATENLI KVIEIKLNAK
RACVSKIDVG PRGVLVSFHN DTPPNIDKLL AYVERLNGVA RLRPDSKLVL QRAWGDPKAR
LHGALQLSKG LAKAAG
//
