ID A0A0Q4G7M3_9SPHN Unreviewed; 835 AA.
AC A0A0Q4G7M3;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN ORFNames=ASE72_04840 {ECO:0000313|EMBL:KQM73902.1};
OS Sphingomonas sp. Leaf20.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1735685 {ECO:0000313|EMBL:KQM73902.1, ECO:0000313|Proteomes:UP000051642};
RN [1] {ECO:0000313|Proteomes:UP000051642}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf20 {ECO:0000313|Proteomes:UP000051642};
RA Garrido-Oter R., Mueller D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000051642}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf20 {ECO:0000313|Proteomes:UP000051642};
RA Vorholt J.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQM73902.1}.
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DR EMBL; LMKN01000001; KQM73902.1; -; Genomic_DNA.
DR RefSeq; WP_056017343.1; NZ_LMKN01000001.1.
DR AlphaFoldDB; A0A0Q4G7M3; -.
DR STRING; 1735685.ASE72_04840; -.
DR OrthoDB; 9802472at2; -.
DR Proteomes; UP000051642; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR CDD; cd04862; PaeLigD_Pol_like; 1.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR014146; LigD_ligase_dom.
DR InterPro; IPR014144; LigD_PE_domain.
DR InterPro; IPR014145; LigD_pol_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014143; NHEJ_ligase_prk.
DR InterPro; IPR033651; PaeLigD_Pol-like.
DR NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR NCBIfam; TIGR02778; ligD_pol; 1.
DR NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR PANTHER; PTHR42705:SF2; MULTIFUNCTIONAL NON-HOMOLOGOUS END JOINING DNA REPAIR PROTEIN LIGD; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF13298; LigD_N; 1.
DR Pfam; PF21686; LigD_Prim-Pol; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:KQM73902.1};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000051642};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 326..452
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
FT REGION 184..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 835 AA; 90663 MW; B3E4592071524AE2 CRC64;
MADTPDPLAR YNAKRDFAKT AEPAGTLTPG NGNSFMVQKH DATRLHWDLR LEIDGVLKSW
AVTRGPSLDP GEKRLAVRTE DHPLSYATFE GTIPAGEYGG GTVMLWDRGS WSPIKGKSAS
DLDKGHLHFV LDGERMKGEW LLIRLKPRAK EKRENWLLRK IDDAAAGGTD TLVDEALTSV
STGRTMAEIA EGKKPARATK PAPPPSSRRK PGSRAKSAAA DTLDPGLWQD DAALPDFRDP
QLCTLVDAVP TGSAWLHEVK YDGYRALVAV ANGTARVFTR TGLDWTDKFA GIAAAAATLP
VRSALIDGEI VAYKDGHPDF STLKDAISNG GDMTLFAFDL LELDGEDLTG LSNLDRKARL
EPLIPGDDNR LRYSDHVVGA GEQLFETMCR EGLEGIVSKR ADAPYAGKRT KAWLKVKCTH
RQEFVIVGWL PSDKKRGFKS LLLGVRDGKG YRYAGKVGTG FDQALMTELR DKLEALDRKT
PTVDAPKAAV RGAKWVTPKL VAEIAFAETT PDGVLRHSSF IGLREDKAAK DVVAETPAPV
PDAADAVPAT RITVTSRDRV LFDESDVTKG DLADYYAAVS GIMLPFAGSR PISLVRCPQG
RSRACFFQKH DAGSFGDAVH QVPIREKDGS TEDYLYVDDA DGLVACVQMG TIEFHGWGSS
VAALEKPDRL VFDLDPDEGL AFSDTKAAAE HLKTQLAELG LVSFPLLSGG KGIHVVVPLT
PDAEWPAVKD FADRFARALA GAEPDRFVAT MAKARRKGRI FIDWLRNQRG ATAVMPYSAR
ARAGAPVAAP VSWTELRDID TAARWGVRDV DELIARVNSR ALKGWGVADQ VLPDL
//
