ID A0A0Q4RJ74_9BURK Unreviewed; 1159 AA.
AC A0A0Q4RJ74;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ASF16_09925 {ECO:0000313|EMBL:KQO18950.1};
OS Acidovorax sp. Leaf78.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Acidovorax.
OX NCBI_TaxID=1736237 {ECO:0000313|EMBL:KQO18950.1, ECO:0000313|Proteomes:UP000051234};
RN [1] {ECO:0000313|Proteomes:UP000051234}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf78 {ECO:0000313|Proteomes:UP000051234};
RA Garrido-Oter R., Mueller D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000051234}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf78 {ECO:0000313|Proteomes:UP000051234};
RA Vorholt J.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQO18950.1}.
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DR EMBL; LMLZ01000021; KQO18950.1; -; Genomic_DNA.
DR RefSeq; WP_056169423.1; NZ_LMLZ01000021.1.
DR AlphaFoldDB; A0A0Q4RJ74; -.
DR STRING; 1736237.ASF16_09925; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000051234; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 625..786
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 807..961
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1159 AA; 129150 MW; 72BACA2DBC7E37CB CRC64;
MELPKLSPGK RFTLPRPVGS ADSLLLARLA ERDKAAGRTT AIVTADATDA QRLIEEMAFF
APGLRCALFP DWETLPYDTF SPHQDLISER LATLWRISQK DKDTGADVVL VPATTALYRL
APPSFLAGYT FHFKVKQKLD EAKFKAQLTL AGYSHVSQVV SPGEYAVRGG LIDLFPMGSL
VPYRVDLFDD EIDSIRTFDP DSQRSLYPVP EVRLLPGREF PMDDDARAKF RSRWREMLEG
DPTKSRIYKD MGAGVATAGI EYYLPLFFDD TATVFDYLGS EATVVLHGDL EPAFQRFWQD
TKDRFRLVQG DPERPVLPPE ELFLSADQFY TRAKEHAQLS IRPGVEDIAE NPHFQKLGDL
SVLRGAEDPL ARLHAHIRNT QHRVLLLAES AGRRESLLDF LRASGVNPPA FDSLAEFQGT
ADEKVGIATA GLTVGFSWIE DGIDFVTETE LFAAGPTTRR RKKQEQVSDV EALIKDLAEL
NVGDPVVHSA HGIGRYRGLV NMDVGNKNPD GTPAMQEFLH LEYADKAVLY VPVSQLQLIS
RYTGVSADEA PLHKLGSGQW EKAKRKAAEQ VRDSAAELLN IYARRAAREG HAFRYSPQDY
ETFANDFGFE ETADQNAAIH AVIQDMISPR PMDRLVCGDV GFGKTEVALR AAFVAVTGGK
QVAFLAPTTL LAEQHYQTLV DRFSKWPVKV AEVSRFRSGK EITAAIKGIG DGTVDIVVGT
HKLLSESTKF HNLGLLIIDE EHRFGVRHKE QMKALRAEVD VLTLTATPIP RTLGMALEGL
RDLSVIATAP QRRLAIKTFV RNEGTGVIRE AVLRELKRGG QVYFLHNEVE TIENRRQKLE
EILPEARIAV AHGQMPEREL ERVMRDFVAQ RYNILLCSTI IETGIDVPTA NTIIMSRADK
FGLAQLHQLR GRVGRSHHQA YAYLMVPDTE GLTKHAAQRL DAIQQMEELG SGFYLAMHDL
EIRGAGEVLG ENQSGNMLEV GFQLYNEMLN EAVKALKAGK EPDLLSPLSV TTDINLHAPA
LLPDDYCGDV HLRLSFYKKL ATAKTPDQID SLLEEIVDRF GKLPPQAQTL IDVHRLRVLS
QPYGVVKVDA APGVINITFK PQPPIDPMRI IELIQKNKHI KLAGNEKLRI ERELKDPKDR
AQMVRDILRS LGQPVAAPV
//
