ID A0A0Q4YK26_9BURK Unreviewed; 1103 AA.
AC A0A0Q4YK26;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=ASF43_02325 {ECO:0000313|EMBL:KQP22757.1};
OS Pseudorhodoferax sp. Leaf267.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae.
OX NCBI_TaxID=1736316 {ECO:0000313|EMBL:KQP22757.1, ECO:0000313|Proteomes:UP000051560};
RN [1] {ECO:0000313|EMBL:KQP22757.1, ECO:0000313|Proteomes:UP000051560}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf267 {ECO:0000313|EMBL:KQP22757.1,
RC ECO:0000313|Proteomes:UP000051560};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KQP22757.1, ECO:0000313|Proteomes:UP000051560}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf267 {ECO:0000313|EMBL:KQP22757.1,
RC ECO:0000313|Proteomes:UP000051560};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQP22757.1}.
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DR EMBL; LMMV01000001; KQP22757.1; -; Genomic_DNA.
DR RefSeq; WP_056176065.1; NZ_LMMV01000001.1.
DR AlphaFoldDB; A0A0Q4YK26; -.
DR STRING; 1736316.ASF43_02325; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000051560; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000051560};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 37..122
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1103 AA; 123614 MW; 16A1DD63C0FB8456 CRC64;
MPDPSHNAQE KQEERAARKW PLPQPVTGAP DPLGLYTELH CLSNFSFQRG ASHPDELVIQ
AYLLGYRGLA LTDECSVAGV VRAYMALKDY RAMLQEAEQA QPGITYQRDF QFLPGSEFAL
PGCRLVVLPH DLVGWGQLCE LISHARRHQH VAKGDYHVDW SDGWGTLTHC EILHVPARHP
APDELLLRTE LRRLRALFGR HLWLAVELVG AADDAQWLDR LRRLSTLTDV PLLAAGGVLM
HARVRKRLQD VLTAVRLGKP VAECGFALEP NAERRLRPRV ALQQFYPPEL LAATETVRER
CQFDLDEIAY RYPRETLPSG LTPTEALRQL TYAEAARLRY PDGMPPGIRA LVEKELSLIA
YCQYEMFFLT VHDIVRFARA EGILCQGRGS AANSVVCYCL GITAANPEDS HPLMERFISK
ERQNEPPDID VDFEHERREE VIQYIYRKYG ADRAAIAAVV ICYRRRSAIR DVGTALGIPA
ALVDAFAKEH HWFDAALIGE RLEQLVDELQ IGIDPRRVAQ WLVLTDQLRG FPRHLSQHVG
GFVLTEGRLT SLVPIEKASM KDRSVIQWDK DDLERMGLMK VDVLALGMLT ALRRCLDFVN
RLRGTQWDLA GIPIKDEATF DMICRADTVG TFQIESRAQM SMLPRLKPRV FYDLVVEVAI
VRPGPIEGGM VHPYLKARER RDKGLPIHYE KEALRPALER TLGVPIFQEQ VMQLAVDAAG
FTAGKADKLR RAMAAWKKEG DITPWHDELV NGMTSRGYTL EFAERIYKQV CGFGEYGFPE
SHAYSFALLA YASSWLKCHE PAAFLAALLN SQPMGFYAPS QLVQDARRSG IAVRAVDVSH
SDWDAALEDA PGVRAQPAAQ GQPAVRLGMR LVKGFNEAAA RRIVAARQAG PFASVEDLSL
RAVLDRRELD ALAAADALAS LAGHRRQQAW EAASRHRAPV LLRGAPVHEP SLALPAAREG
EEILFDYDAT GLTLRRHPLA LLRERLARSR ILNADQLRDL PHGRRVRAAG LVTVRQQPGT
ANGTIFVTLE DETGPVNVIV WPRVRELQRA PLMNARLLAV EGLWQRDVDS GGQVQHLVAE
RLKDLTPLLG RLGQLRSRSR DFH
//
