ID A0A0Q5APC7_9MICO Unreviewed; 889 AA.
AC A0A0Q5APC7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=ASF42_12830 {ECO:0000313|EMBL:KQQ01363.1};
OS Rathayibacter sp. Leaf294.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Rathayibacter.
OX NCBI_TaxID=1736326 {ECO:0000313|EMBL:KQQ01363.1, ECO:0000313|Proteomes:UP000051858};
RN [1] {ECO:0000313|Proteomes:UP000051858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf294 {ECO:0000313|Proteomes:UP000051858};
RA Garrido-Oter R., Mueller D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000051858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf294 {ECO:0000313|Proteomes:UP000051858};
RA Vorholt J.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQQ01363.1}.
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DR EMBL; LMNO01000004; KQQ01363.1; -; Genomic_DNA.
DR RefSeq; WP_056042828.1; NZ_LMNO01000004.1.
DR AlphaFoldDB; A0A0Q5APC7; -.
DR Proteomes; UP000051858; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000051858};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 6..268
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 645..852
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 889 AA; 96944 MW; F3A1FB1DF6667B5C CRC64;
MSDPEQPTLL LIDGHSLAFR AFYALPVDSF QTRDGQHTNA IHGFLSMLIL LLKNEKPTHL
GVAFDISRYS FRTREYPEYK GTRGETPPEF KGQVPLLEEA LHAMNIRTVS KEDFEADDIL
ATLAKQGAEA GFRVLVVSGD RDTIQLVTPD VTLLYPSTQG VSQLTRYDRD KVFEKYGVEP
HQYPEIAALV GETSDNLPGV DKVGPKTAAK WVNQYGTVAD IIAHADEIKG VVGEKLREQK
ENALRNRRLN RLVTDVELPL GPADLDRRPI DVDAVREIFS RLEFKTLQER VLTIAADEGV
EGAASALAGD TVTTAAPAVR TLVDEELAHW LDRVSAKGTT PVAVQVQLGP DGLDGLGLST
ETESAFVPWA AGRPDYTALE EWLAGEAPKV FADAKPQIKA LRRAGLTVSG LAFDTRVAAY
LLQPTGHAAT LPAQVAQYLD ETMAQGDPNQ LVPSVEPIGP ATEAWYAFRL VAPLTELLGE
GMLSVLVDIE LPIVQVLATM ELTGVSIDSD VLARLRGELT TTAAELASRA FAEIGHEMNL
GSPKQLQQVL FEELAMPKTR ATKTGYTTDA SALADLQDSH PHPFLGLLLE HRDATKLKQI
IETLEKSVTA DQRIHTSYDQ TGTATGRISS NDPNLQNIPV RSEVGRRIRT AFVHGEEAST
LLTADYSQIE MRIMAHLSGD AGLIEAFHSG EDLHRFVGAR IFGVEPADVT SAMRNKVKAM
SYGLAYGLSA FGLSKQLRIS SAEAKQLMTD YFVRFGAVRD YLRSVVEQAR EAGYTETIFG
RRRPFPDLQS ANRVLRDNAE RAALNSPIQG SAADIMKIAM IGVQADIDEQ GLGSRMLLQV
HDELIFDVSP GEEEALEAVV RARMAGAVDL SVPLEVQVGH GADWDDAAH
//
