ID A0A0Q5CFA2_9MICO Unreviewed; 1207 AA.
AC A0A0Q5CFA2;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ASF54_12220 {ECO:0000313|EMBL:KQQ26726.1};
OS Frondihabitans sp. Leaf304.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Frondihabitans.
OX NCBI_TaxID=1736329 {ECO:0000313|EMBL:KQQ26726.1, ECO:0000313|Proteomes:UP000051456};
RN [1] {ECO:0000313|EMBL:KQQ26726.1, ECO:0000313|Proteomes:UP000051456}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf304 {ECO:0000313|EMBL:KQQ26726.1,
RC ECO:0000313|Proteomes:UP000051456};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KQQ26726.1, ECO:0000313|Proteomes:UP000051456}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf304 {ECO:0000313|EMBL:KQQ26726.1,
RC ECO:0000313|Proteomes:UP000051456};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQQ26726.1}.
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DR EMBL; LMNV01000003; KQQ26726.1; -; Genomic_DNA.
DR RefSeq; WP_055963286.1; NZ_LMNV01000003.1.
DR AlphaFoldDB; A0A0Q5CFA2; -.
DR STRING; 1736329.ASF54_12220; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000051456; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000051456}.
FT DOMAIN 663..824
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 849..999
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1207 AA; 131438 MW; 85F392E577FA839F CRC64;
MILQGLIPAL SRASTFDSAL KQATRSSDFS LVDGLRVPLL AGLLDRRAEG SGAAQALLVI
TATGRESEAV RTSLTSYLPG AEVIEFPAWE TLPHERLSPS AETVGKRIHA LRRLVEWQSA
FASDPSSVAP LVVVAGVRAA LQPISDNLTD IDAVRLTVGE RGYDLSAIAV RLVDLAYARV
DMVTRRGEFA VRGGILDVFP PMAEHPVRVD FFGDEIDQIR LFAVADQRSL DTEAKTVELP
PSREMLLTES VRQRAREMQH EFPSLAQMLE KIGQGIPVDG MESLAPALVH RLVPLTHYLP
PSAAIAVLSP ERVVSRAKSL ADTNREFLQA AWSAATAGAE APIDLDSGDF LTVGDLRLQA
GDRTWWSLSG FDSGVVLSDE ERIESAGDYV RIDAETVPSF SGNAEGAVEH VAELAAAGWS
IVVTARGVGL VDRARDVLAE RGVAARIVEA LGDDLEPGVV HLVTGGVEAG FQVPEAKLAV
LGEADFFGRA AGYDGRQVKK LASRRKNVVD PLQLKTGDIV VHSTHGIGRF LELTSREVSS
GGRNAVKHSR EYLVLEYAPS KRGQTGDRLY VPTDQLDLLS RYVGGEAPKL SKMGGSDWSQ
AKGKARKAVR DIAVELVKLY SARMASRGHA FGPDTPWQRE LEEAFPFAET PDQLTTIDEI
KADMERPIPM DRLISGDVGY GKTEVAIRAA FKAIQDGKQV AMLVPTTLLV KQHMETFSER
FAGFPVHLRA LSRFQTAKES KETIKGIGDG TVDIVIGTHR ILTEGLSFKD LGLVIIDEEQ
RFGVEHKDQL KKLKTNVDIL AMSATPIPRT LEMAVTGIRE MSTLATPPED RHPILTFVGP
NSERQIGAAI RRELLREGQI FFVHNRVSSI NRVAAQLAEL VPEARVAIAH GQMPESALEQ
VMVDFWERKF DVLVSTTIIE TGLDIANANT LIIDRADRYG LSQLHQLRGR VGRGRERGYA
YFLYDADKPL SETAQDRLET IAANNELGAG MQVALKDLEI RGAGNLLGGE QSGHIAGVGF
DLYLRMIGEA VNAFRGDVAE GQTELRLELP VDAHIPEDYV ESERLRLEAY QKLSTASSPT
SSDEQIDSVL EELTDRYGEP PAEVLALIEV SRLRRMAQKT GLSEVVAMGT NMRVAAMELA
DSVQLRLARL FPGARYFAQT KSVSVPMPRK NGEALPDAEL ITWVEQLLTS VYGAEPARPA
DSAAAPS
//
