ID A0A0Q7ALP7_9BURK Unreviewed; 1038 AA.
AC A0A0Q7ALP7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273, ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=ASC76_17970 {ECO:0000313|EMBL:KQW36548.1};
OS Rhizobacter sp. Root404.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales; Rhizobacter.
OX NCBI_TaxID=1736528 {ECO:0000313|EMBL:KQW36548.1, ECO:0000313|Proteomes:UP000051611};
RN [1] {ECO:0000313|EMBL:KQW36548.1, ECO:0000313|Proteomes:UP000051611}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root404 {ECO:0000313|EMBL:KQW36548.1,
RC ECO:0000313|Proteomes:UP000051611};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KQW36548.1, ECO:0000313|Proteomes:UP000051611}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root404 {ECO:0000313|EMBL:KQW36548.1,
RC ECO:0000313|Proteomes:UP000051611};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391, ECO:0000256|HAMAP-
CC Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQW36548.1}.
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DR EMBL; LMDS01000005; KQW36548.1; -; Genomic_DNA.
DR RefSeq; WP_056469456.1; NZ_LMDS01000005.1.
DR AlphaFoldDB; A0A0Q7ALP7; -.
DR STRING; 1736528.ASC76_17970; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000051611; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000051611};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1038 AA; 116938 MW; 8FFEF0C9C45FCF5D CRC64;
MNIPEYAELR CVSNFTFLRG ASQPEELVER AKQLGYSALA VADECSLAGI VRAHVAAKEQ
DFKLLVGAQF QVDWGVATSS STTRFTLTVL ACNLFGYGNL CQFITKLRRS SEKGTYDLDI
ADITGSELEE CVVIMSPQRM AEVEQLQTAA KWLLDHFIGR CWLGVEQIRV LDDEMWLHRL
RQVSGSTAIP LVAAGDVHFH VRSRKPLQDV LTATRIGKPL TECGLDLQPN AERHLRTRLR
LAQTFPEDLL AETLVVAARC VFSLEDLRYQ YPDEVVPTGH TSSSYLRQIT YEGAGRRWPQ
GMPAKVQLQI EHELELIKDL KYEHYFLTVA DIVAFARSRH ILCQGRGSAA NSVVCYCIGV
TEVDPARMSV LFERFISKER KEPPDIDIDF EHERREEVLQ YLYRKYGRDR AAITGVVTSY
RPKSAIRDVG KALGYSLETV DAIAKGHQWW DGREVKAERL TEVGLSIDDL QVRQLLHLTE
QLLGFPRHLS QHTGGFVLTK GPLSRMVPIE NASMEDRTVI QWDKDDLDVM GLLKVDCLAL
GMLTAIRKSL EFIGARKGYQ FEMQDIPAED PQTYDMVCKA DTVGVFQIES RAQMSMLPRM
KPRCFYDLVI EVAIVRPGPI VGGMVHPYLN RRQGKEPVTY PKEDLKEALG RTLGVPVFQE
QVMQISIIAA GFTPGEADQL RRAMAAWKRK GGLEKYHSKI VDGMTSRGYE KEFAEGIFKQ
IHGFSEYGFP ESHAASFALL VYASCWIKRH HPAEFLAALL NSQPMGFYSP SQLVQDAKRH
GVEVRRPDVM ESDVDCTLED LPHPPAVRLG LRMIAGLKWE SAERIVAARR VRVFDNAEDM
ARRAEVELHE MKLLAAADAL MSLSGHRRQQ VWDAAALRST PELLRDAPFD EEVLELPSAP
EGEEVVFDYV SLGLTLRTHP MGLLRPHLAG RHLKTAEDLD AARSGQLVHY AGIVTLRQQP
DTANGTVFIS LEDETGVVQV ICWKSLRDAQ RKELLRSRLL LVHGTWQREG EVKSLIAGKL
EDLTPMLGRL TTESRDFK
//
