ID   A0A0Q7B8K3_9ACTN        Unreviewed;       349 AA.
AC   A0A0Q7B8K3;
DT   20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT   20-JAN-2016, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=3-hydroxyisobutyryl-CoA hydrolase {ECO:0000256|ARBA:ARBA00011915};
DE            EC=3.1.2.4 {ECO:0000256|ARBA:ARBA00011915};
GN   ORFNames=ASC77_19725 {ECO:0000313|EMBL:KQW45016.1};
OS   Nocardioides sp. Root1257.
OC   Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC   Nocardioidaceae; Nocardioides.
OX   NCBI_TaxID=1736439 {ECO:0000313|EMBL:KQW45016.1, ECO:0000313|Proteomes:UP000051939};
RN   [1] {ECO:0000313|EMBL:KQW45016.1, ECO:0000313|Proteomes:UP000051939}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Root1257 {ECO:0000313|EMBL:KQW45016.1,
RC   ECO:0000313|Proteomes:UP000051939};
RA   Gilbert D.G.;
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:KQW45016.1, ECO:0000313|Proteomes:UP000051939}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Root1257 {ECO:0000313|EMBL:KQW45016.1,
RC   ECO:0000313|Proteomes:UP000051939};
RA   Schulze-Lefert P.;
RT   "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL   Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-
CC         methylpropanoate + CoA + H(+); Xref=Rhea:RHEA:20888,
CC         ChEBI:CHEBI:11805, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57340; EC=3.1.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00001709};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KQW45016.1}.
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DR   EMBL; LMDV01000012; KQW45016.1; -; Genomic_DNA.
DR   RefSeq; WP_056157936.1; NZ_LMDV01000012.1.
DR   AlphaFoldDB; A0A0Q7B8K3; -.
DR   STRING; 1736439.ASC77_19725; -.
DR   OrthoDB; 9790967at2; -.
DR   Proteomes; UP000051939; Unassembled WGS sequence.
DR   GO; GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:InterPro.
DR   CDD; cd06558; crotonase-like; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR045004; ECH_dom.
DR   InterPro; IPR032259; HIBYL-CoA-H.
DR   PANTHER; PTHR43176:SF3; 3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43176; 3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED; 1.
DR   Pfam; PF16113; ECH_2; 1.
DR   SUPFAM; SSF52096; ClpP/crotonase; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KQW45016.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000051939}.
FT   DOMAIN          21..340
FT                   /note="Enoyl-CoA hydratase/isomerase"
FT                   /evidence="ECO:0000259|Pfam:PF16113"
SQ   SEQUENCE   349 AA;  37237 MW;  84B524CD3B0EC7A8 CRC64;
     MSDNAGTDDR PVLVEQRGRL GVLTLNRPRA INALTHEMVG ILDHALRQWA GDDTVETVLL
     RGAGDRGLCA GGDIVSIHRD AVTGGRGSED FWRDEYALNS LISGYPKPYV ALMDGIVLGG
     GVGVSAHASH RIVTERTRIG MPETGIGFVP DVGGTWLLSH APGELGTYVA LTAGHVDAGD
     ALALGLADHH VLAADLPRLV ELLTDRPVDA ALGHVVQPVP QSRLSEIRAW VDASFASDDL
     SAILDRLAAS DDPAAEPARE ALARNSPTSL AVTLRALRTA RGMSGLDEVL DQEYRIAVRM
     LRGHDFPEGI RAQVIDKDRN PRWEPATVAG LDPAAIDAYF APLFQEESA
//