GenomeNet

Database: UniProt
Entry: A0A0Q7ZQF6_9ACTN
LinkDB: A0A0Q7ZQF6_9ACTN
Original site: A0A0Q7ZQF6_9ACTN 
ID   A0A0Q7ZQF6_9ACTN        Unreviewed;       374 AA.
AC   A0A0Q7ZQF6;
DT   20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT   20-JAN-2016, sequence version 1.
DT   05-DEC-2018, entry version 17.
DE   RecName: Full=Alanine dehydrogenase {ECO:0000256|PIRNR:PIRNR000183};
DE            EC=1.4.1.1 {ECO:0000256|PIRNR:PIRNR000183};
GN   ORFNames=ASD66_13210 {ECO:0000313|EMBL:KQZ70552.1};
OS   Nocardioides sp. Root151.
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Nocardioides.
OX   NCBI_TaxID=1736475 {ECO:0000313|EMBL:KQZ70552.1, ECO:0000313|Proteomes:UP000051274};
RN   [1] {ECO:0000313|EMBL:KQZ70552.1, ECO:0000313|Proteomes:UP000051274}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Root151 {ECO:0000313|EMBL:KQZ70552.1,
RC   ECO:0000313|Proteomes:UP000051274};
RA   Millard Andrew;
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:KQZ70552.1, ECO:0000313|Proteomes:UP000051274}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Root151 {ECO:0000313|EMBL:KQZ70552.1,
RC   ECO:0000313|Proteomes:UP000051274};
RA   Vorholt J.;
RT   "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL   Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-alanine + NAD(+) = H(+) + NADH + NH4(+) +
CC         pyruvate; Xref=Rhea:RHEA:18405, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:57972;
CC         EC=1.4.1.1; Evidence={ECO:0000256|PIRNR:PIRNR000183};
CC   -!- PATHWAY: Amino-acid degradation; L-alanine degradation via
CC       dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step
CC       1/1. {ECO:0000256|PIRNR:PIRNR000183}.
CC   -!- SIMILARITY: Belongs to the AlaDH/PNT family.
CC       {ECO:0000256|PIRNR:PIRNR000183}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KQZ70552.1}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; LMGG01000004; KQZ70552.1; -; Genomic_DNA.
DR   RefSeq; WP_056891218.1; NZ_LMGG01000004.1.
DR   EnsemblBacteria; KQZ70552; KQZ70552; ASD66_13210.
DR   UniPathway; UPA00527; UER00585.
DR   Proteomes; UP000051274; Unassembled WGS sequence.
DR   GO; GO:0000286; F:alanine dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0042853; P:L-alanine catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05305; L-AlaDH; 1.
DR   InterPro; IPR008141; Ala_DH.
DR   InterPro; IPR007886; AlaDH/PNT_N.
DR   InterPro; IPR007698; AlaDH/PNT_NAD(H)-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR42795; PTHR42795; 1.
DR   Pfam; PF01262; AlaDh_PNT_C; 1.
DR   Pfam; PF05222; AlaDh_PNT_N; 1.
DR   PIRSF; PIRSF000183; Alanine_dh; 1.
DR   SMART; SM01002; AlaDh_PNT_C; 1.
DR   SMART; SM01003; AlaDh_PNT_N; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR00518; alaDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000051274};
KW   NAD {ECO:0000256|PIRNR:PIRNR000183, ECO:0000256|PIRSR:PIRSR000183-3};
KW   Nucleotide-binding {ECO:0000256|PIRSR:PIRSR000183-3};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000183};
KW   Reference proteome {ECO:0000313|Proteomes:UP000051274}.
FT   DOMAIN        4    140       AlaDh_PNT_N. {ECO:0000259|SMART:SM01003}.
FT   DOMAIN      152    300       AlaDh_PNT_C. {ECO:0000259|SMART:SM01002}.
FT   NP_BIND     242    243       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   NP_BIND     270    273       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   NP_BIND     301    304       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   ACT_SITE     99     99       Proton donor/acceptor.
FT                                {ECO:0000256|PIRSR:PIRSR000183-1}.
FT   ACT_SITE    273    273       Proton donor/acceptor.
FT                                {ECO:0000256|PIRSR:PIRSR000183-1}.
FT   BINDING      15     15       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000183-2}.
FT   BINDING      78     78       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000183-2}.
FT   BINDING     137    137       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   BINDING     201    201       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   BINDING     206    206       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
FT   BINDING     223    223       NAD. {ECO:0000256|PIRSR:PIRSR000183-3}.
SQ   SEQUENCE   374 AA;  39109 MW;  5DDE1D1BC5D84C64 CRC64;
     MKVGVPKEVK NHEYRVAITP IGVHELTAHG HEVVIQQGAG VGSSISDEEY VAAGATIAAT
     AEEAWGTDGG VDLVLKVKEP VAEEYHRMRE GQLLFTYLHL AADKPLTDEL LARKVTGIAY
     ETVQLPSGGL PLLYPMSEVA GCLAPQVGAH SLLKAQGGRG VLMGGVGGVA NAKVVIIGAG
     VSGQNAANIA LGMGADVTLL DTDLDKLRMS FWRYNNRVHG LASSKLAIEQ QVMEADMVIG
     AVLIPGAAAP KLVSNDLVSR MKPGSVLVDI AVDQGGCFED THATTHADPT YQVHQSTFYC
     VANMPGAVPN TSTYALTNAT LPYAVALADK GWHQACRDDH SLALGLNTHD GRLTNKPVGE
     ATGIAAVSPD EVLG
//
DBGET integrated database retrieval system