ID A0A0Q8EHG4_9ACTN Unreviewed; 1072 AA.
AC A0A0Q8EHG4;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=ASD81_04690 {ECO:0000313|EMBL:KRA39361.1};
OS Nocardioides sp. Root614.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=1736571 {ECO:0000313|EMBL:KRA39361.1, ECO:0000313|Proteomes:UP000051699};
RN [1] {ECO:0000313|EMBL:KRA39361.1, ECO:0000313|Proteomes:UP000051699}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root614 {ECO:0000313|EMBL:KRA39361.1,
RC ECO:0000313|Proteomes:UP000051699};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KRA39361.1, ECO:0000313|Proteomes:UP000051699}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root614 {ECO:0000313|EMBL:KRA39361.1,
RC ECO:0000313|Proteomes:UP000051699};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRA39361.1}.
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DR EMBL; LMGV01000001; KRA39361.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0Q8EHG4; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000051699; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000051699}.
FT DOMAIN 5..346
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 347..660
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 779..800
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1072 AA; 115374 MW; 3A8D8F9B70F1E19E CRC64;
MQAKFPPSEE QWAAISAPLR PAVVIAGAGS GKTTLMAARV VYLVLTGQVR PEEVLGLTFT
TKAAAELRQR IRGALRDAGA LDLGATGGDE ADEVLEPTVA TYNAYAATLL TDHGLRIGHE
PDTRVITDAA RYQLGARAID RFTGDIEHLT DHPETAIQNL LALDSAMSEH LVAPAQVLAH
DEEARRGFER ARAEELAGKA RKTYVEVIEK AMSAIDRRAD LLKLVASYRG LKRDLGLMDF
SDQIELGARL AAERPEVGAV ERGRFKVVLL DEYQDTSVAQ AIMLSRLFSG EVDGRGHAVT
AVGDPNQAIY GWRGASVANI LNFAETFPAA DGDSRTHPLT INRRSDRRIL DVASRLAAPL
YDALEARGAG VARLRAPENA ADGLVTAEVF ETQRDELAWL AASIRAGWGG EPGDWARVGV
LTRDNAHAEL VFDALTSANI PVEIVGLSGL LRLPEIAEIV ATLRLLHDVT DNAALLTLLA
GPRWAIGPRD LRLLGRRAAE HAGRRGRSSD AVSVSAQLVA IADGIDPAEI PCLDDALADP
GDAEYSQEAL ARFGLLRDEL RLLRSAVGEP LLDLVRRIID VTGVDVELAS ATSPAAAARR
DNLDLFVKAV ADFQAVDGDV TLPALLAYLT AEDDQGNGLD VATPTEADSV KLLTVHRSKG
LEWDTVYCVG VGESRFPSSQ GRTLWVSSPS VLPAPLRGDA ADLPQLAGFD KAALEDYRQR
AKAHEADEEL RLGYVAFTRA AHHLSVTSYQ WGPRKTPYGP SSYQETVREL LGEWGLAPDP
WLTRPEKGSP NPYDAEDPSR PWPADGFGEE ARLRIAAAEV VRAADPEAED TDLDLVASAR
VAEWDAEIDQ LLAEARVQAA GGAGTEVVVT LPASLSATAM GRLRDDPEGL ARDLARPMPR
PPAPAARFGT RFHAWVEARF GQQGLFDPDE LAGRADAGID DEADLQELIA TFEAGPFGSR
VPHAIEAPFA LVLGTQGGAQ QLVRGRIDAV YVEPDGGDRA GAGGPSYLVV DWKTNARQDA
DPLQLALYRL AWAELHDLPL EQVRAAFYYV RSGDLVVHDD LADRDEIEKL LG
//
