ID A0A0Q8LYK7_9MICO Unreviewed; 1201 AA.
AC A0A0Q8LYK7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ASD93_03775 {ECO:0000313|EMBL:KRB39210.1};
OS Microbacterium sp. Root180.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1736483 {ECO:0000313|EMBL:KRB39210.1, ECO:0000313|Proteomes:UP000050802};
RN [1] {ECO:0000313|EMBL:KRB39210.1, ECO:0000313|Proteomes:UP000050802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root180 {ECO:0000313|EMBL:KRB39210.1,
RC ECO:0000313|Proteomes:UP000050802};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KRB39210.1, ECO:0000313|Proteomes:UP000050802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root180 {ECO:0000313|EMBL:KRB39210.1,
RC ECO:0000313|Proteomes:UP000050802};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRB39210.1}.
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DR EMBL; LMHS01000001; KRB39210.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0Q8LYK7; -.
DR STRING; 1736483.ASD93_03775; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000050802; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000050802}.
FT DOMAIN 656..817
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 842..992
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1201 AA; 129897 MW; FAFAB14A45FE0F37 CRC64;
MRALEQASSF QDAAAAASVD GDFSLVEGLD APLLAALLER RRAAGRPPAI LLITPTGRRA
ESIGPALGAL LPGAEVLHFP AWETLPHERL SPSPEIVGNR LEVLGRVGRW SGETPLVVTA
SVRGAIQPLA AGLTDVAPID LAVGERGHEL GDVVLRLVEL AYHRVDMVSR RGEFAVRGGI
LDIFPPTAAH PYRIEFFGDE VDQIRAFSVA DQRSLPGEVR EVSLIASREL LLTEAVRGRA
RQLKDEFPSL AGMLERMSEG IPVEGMESLL PAVSDRLVTL VDYLPEGSAV ALVDPERAVA
RAITLGETNR EFLEAAWSAA TAGASTPIDL GAGDFLTLPA LREAARARRQ VWWSLSAFDS
GEADAAAEGL LDIDSALEAA AAIRVPGTAV PSFHGNVDGA TEHVGALLAD GWRVVVAASG
TGLVERARDV LAERGIAARK VGDIHSAPEP GVAHLVTSVL ERGFEAADAK LAVLTETEFY
GRTIGGDQRV VKKLASRRKN VVDPIQLKAG DHVVHVTHGI GRFVELQQRE VSSGGRNPVK
SVREYLVIEY APSKRGFPGD KLLVPTDQLD LLSRYVGGEA PALSKMGGSD WAAAKGRARK
AVRDIAVELV KLYSARMAAK GYAFGPDTPW QRELEEAFPF AETPDQLQTI DEVKADMEKP
VPMDRLLSGD VGFGKTEVAV RASFKAIQEG KQVAMLVPTT LLVKQHIETF TERFAGFPVK
VKALSRFQTD KQAREVVAGL ADGTVDMVIG THRILTEKVL FKDLGLMIID EEQRFGVEHK
DTLKKLKTNV DILAMSATPI PRTLEMAVTG IREMSTLQTP PEDRHPILTF VGPRNDKQIA
AAIRRELLRE GQIFYVHNRV QSIQRIATDL AELVPEARIA VAHGQMGEHQ LEQVVDDFWE
RRADVLVSTT IIETGLDIAN ANTIIIDRAD KYGLSQLHQL RGRVGRARER AYAYFLYDEL
KPLSETAADR LETIAVNNDL GSGIQVALKD LEIRGAGNLL GAEQAGHIAG VGFDLYLRMI
GEAVSAFRGD DVEGPAELRL ELPVQARIPD SYIDSERLRL EAYQKLSAAA SVTAKDDALD
LVVEELSDRY GEPPADVEGL LAVARLRRRA AQTGLTDVVA MGPNLRIAPA HLPDSMRVRL
QRLYPKAKLL SGGDALVVPL PTAGGEALAD ADLIAWVRQL LDQLWPEKKA EAPAADGAPT
P
//
