ID A0A0R0G643_SOYBN Unreviewed; 2168 AA.
AC A0A0R0G643;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN ORFNames=GLYMA_15G048100 {ECO:0000313|EMBL:KRH10453.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRH10453.1};
RN [1] {ECO:0000313|EMBL:KRH10453.1, ECO:0000313|EnsemblPlants:KRH10453}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH10453};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH10453.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH10453}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH10453};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH10453.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH10453.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR EMBL; CM000848; KRH10453.1; -; Genomic_DNA.
DR PaxDb; 3847-GLYMA15G05350-1; -.
DR EnsemblPlants; KRH10453; KRH10453; GLYMA_15G048100.
DR Gramene; KRH10453; KRH10453; GLYMA_15G048100.
DR OMA; EDETRCH; -.
DR Proteomes; UP000008827; Chromosome 15.
DR ExpressionAtlas; A0A0R0G643; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827}.
FT DOMAIN 1922..2016
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
SQ SEQUENCE 2168 AA; 243454 MW; D0374CF639BD95D5 CRC64;
MASPSESSLI SKLQSSDSPG IHALVSDYLR PLSDLKPTKK SNPDQTLIRS LAKRFLSFLN
ASLSILPKRL PEVSKSNNAV SLLELLRVYR LCLDCLDAVA SQLASKPFSV EFQRLRLIHC
LESCGLFDEA QLEGLGVLEK LPPAKRKGKL LPEIDKGSGE VCPKMISIMH RRMIPYMKKL
LPVSSFVLRL DSNSYEKLHK MLVTHLGKCT LNLLGRTPFP DRDLVTLFWC TTLTEYVKSP
IKDQVYKIAL RVCSSLFALR DNNSLYIMDI LDSIVRECKV EEGNTGTDFV ELVYYCANKC
QTANASFCST FAAYLNKIAE HFKQVMTPIN SILRLYAAGL LLVSCNLRSR TGDLASSGSA
KFECLLGTLL ENEKILQNSP PLLGSLHICS KSNCMSSSVE DQCFAGHPCA LSGFDGEASM
TYLSGYLEAL KYLCQPLAKS INSERKELVT KVDDASAMTM MSTVQDAFHI LCHLILSSTS
SMPKNNGDGF DEKSKTVLNL VVAAFTLSIR TNLKVQESKQ LINQIIASKW IEAEGLKYII
ACLYNIAVVL YRNRLLKKAS KVLNLCCKAS WLCIKYHSAN LSEGALKEFV MEACTRSALL
LDIIHDVNNL KINKKMIDIL KNWFTANDLF EGLPAPIPVV KQWVKIECKR ATQVDERIDS
LTLYSLLSSS LELSKRNIAM ILEQELTAYE EMGFKYPEFC QKMQMKITDI LLQDIYITLD
SRFQKAQTLV RKGKAIRVRG IGGLRDCIQC LSEAITIMNE IFGEICTDNN PIHHQLCVTY
CLRAICTQEA EANSKQIFED VKAALDLWLS ISHLNRFEEG DSSALSDNIM ILLYNVVDLL
QLKGFMELFN DAYQLLIKMF KRKNVPIEKW LPLMWESRSL SHALCVSPVN EEFIMNSLDE
FSELSNIDFW IRYLQGNQSS LIGFQQNFSF LFASSHRNSC SHGSSFQTDI TIDEVQKAAL
DLISNVPVPS HSTFLAGCLF YDLCPRLVAN GQLIEALSSA KEAHRLHAKL FQRKFTHNVQ
QQNEEKNVMV DSSKNLMDGV ENIGLNLSVA REVLLFDSIS WDLKDSYLSA WKVMQCYLES
TLQLGIIHEM IGDGAEAETY LKWGKAISCS LKLPFFIIIF SSSLGKLYLK KRLWDLAEKE
LLSAEQILKE SNTPFCCSKC KLKLEVTLYE YLGDLCQSKF DTCDGVMSEE TAKNWYTSAL
DKLNLSEWKN PLSCPEDGSD GTATDIKCPA GKTCTCFIMN EVGESVKKSM KAGRETEIGA
KQNRKTKNAA KVLPKEPNLV VENKSRLTRS RYRSSQNQYT SIHGKLEVHE SLEGNHVSDS
SDMLSQNGSV LSNIGCTFAS RCAITCVFSK MKCWYCLPSE VVKSGLLNDF INLKWEFVRR
QHSMKLLSRV VKCFAYPGQI DEARKILLRS ISVLVSRNPF YPMPSSIPLD YFLHLVAREI
PGDVFTIERA EIVHDICWYS LKNYHSKFAR NIFCNLSFIK FEDLASWLMV AFVLSREVPV
IFQKVSKLLA VMCVVSSLSE QFSLSSFSKA LGENYWSSYF HQASIGTHLT YQFLSHLTGR
CKGSYVTGSS SIKECTSDLL RLVPDTTVDL AEYVKKFLAG LPSTTIISIS LLGRDYTSLL
QELLSYPTCV QAWMLVSRLS FKNEPVVMLL PLDSILQASC EGDLSTGSGT SPKCEEHSEK
WHCPWGFTVV DDVAPTFKTI LEENYLSTKS PLEDTTQNRM LWWKRRKNLD HCLDKLLRNL
EESWFGSWKC LLLGEWLNCK NFDLVLKNLV NDLRSKCKLD VNEGLLKIIL GGSKYVCDGK
TLVSQLCSKK DCYIAKVGYC DEARRGILNS ANGIGVSSEV AFELLSEALN VLEVDDSVNR
EPIILVLDYE VQMLPWENLP ILRNQEVYRM PSVSCISAVL HKGSNHKEQV GRNLGPFPLI
DPLDAFYLLN PDGDLGGTQI EFENYFRDQN LEGKAGLRPT IKELASALES HDLFIYFGHG
SGGQYIPRHE IQKLDKCAAT LLMGCSSGSL TLPGQYAPQG IPLSYLLAGS PAIVGNLWEV
TDKDIDRFGK AMLDAWLKER SDMPTECLQC NLLSEEFEAM NLKGCKGRAK RKAPRKKLLE
LAESESPKNC GHRRKIGAFM GQAREVCTLP FLTGASPVCY GVPTGIWRKG MCSLTNPTQE
SLPQPLLV
//
