ID A0A0R1HS45_9LACO Unreviewed; 748 AA.
AC A0A0R1HS45;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=FC66_GL000695 {ECO:0000313|EMBL:KRK46194.1};
OS Dellaglioa algida DSM 15638.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Dellaglioa.
OX NCBI_TaxID=1423719 {ECO:0000313|EMBL:KRK46194.1, ECO:0000313|Proteomes:UP000051450};
RN [1] {ECO:0000313|EMBL:KRK46194.1, ECO:0000313|Proteomes:UP000051450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15638 {ECO:0000313|EMBL:KRK46194.1,
RC ECO:0000313|Proteomes:UP000051450};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRK46194.1}.
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DR EMBL; AZDI01000002; KRK46194.1; -; Genomic_DNA.
DR RefSeq; WP_057973770.1; NZ_AZDI01000002.1.
DR AlphaFoldDB; A0A0R1HS45; -.
DR STRING; 1423719.FC66_GL000695; -.
DR PATRIC; fig|1423719.4.peg.705; -.
DR Proteomes; UP000051450; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF29; PENICILLIN-BINDING PROTEIN 1A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645,
KW ECO:0000313|EMBL:KRK46194.1}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000051450};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..45
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 77..247
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 344..590
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 748 AA; 82661 MW; 95561BB994F38866 CRC64;
MEKQEKIKKT KWRYRHPHIF RYVAIFSVLL LLGVAAISLY VAAVIKSVPT VTSQELISDA
SSNMYDREGQ LIWSSALNKR VYVKAKDIPK IYSDLLLSTE DQTFYKDAGF SPSGLANAAF
STVKSAVGKG EVRGGSSIEQ QLIKLTVFST SNKDRTVDRK IKEFFLAQQL YHNYSKDQIL
EFYINKMSLG EGSYGAQTIS ETYFGKTLKQ LSVSQQAIIA GLGQAPSTYN LYSNPKAVRA
RRNIVLKTGL ENKVITKSQY KIAIAESVTK DLKKRHWMEN SVTKKILENN AYVTSALEQV
SDKGYDLSKT PLQINTALDS SKNAYVKKLA DDEKYYQDST EQSAITITDP KTGNIVAQYG
GRHQKTAFSY NRAISTQRSS GSGIKPLLDY GPAIEYFGWA TTHALDGTAY AYRGTNLLAQ
NFDDVSYRSI TMQNALRVSA NTPAIRTLDT VGSAKAAQFL TQIGMPQTNA PAGSQAIGLD
VSTEQMATAY GAVSNGGTYH AARYVTSLKF SDNSVKKYDV IGKRAMKAST AFILTSMMEG
VPNAGGTAPN AKINGIHQAM KTGTVGYSAA AKFPENANMD VWMNGFTKST SVSMWLGYDE
PMKKGHYITD AASAMTNVNL YRDLMEHFSK ENDADNSEWE KPSTVNQLSG IGLSAQYEPN
DKVVVLGVKE PETIDLKKAK VYKTYLSKTI DKDILKVKFE DIAYQKKPDS YKIGSWKKDT
NDKLDLTQKK LDKANDGIKS AKEMLEGE
//
