ID A0A0R1HT68_9LACO Unreviewed; 499 AA.
AC A0A0R1HT68;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Citrate lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE Short=Citrase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE EC=2.8.3.10 {ECO:0000256|PIRNR:PIRNR009451};
DE EC=4.1.3.6 {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate (pro-3S)-lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate CoA-transferase subunit {ECO:0000256|PIRNR:PIRNR009451};
GN ORFNames=FC96_GL001064 {ECO:0000313|EMBL:KRK48745.1};
OS Secundilactobacillus kimchicus JCM 15530.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Secundilactobacillus.
OX NCBI_TaxID=1302272 {ECO:0000313|EMBL:KRK48745.1, ECO:0000313|Proteomes:UP000050911};
RN [1] {ECO:0000313|EMBL:KRK48745.1, ECO:0000313|Proteomes:UP000050911}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 15530 {ECO:0000313|EMBL:KRK48745.1,
RC ECO:0000313|Proteomes:UP000050911};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089; EC=4.1.3.6;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR009451}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRK48745.1}.
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DR EMBL; AZCX01000002; KRK48745.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0R1HT68; -.
DR STRING; 1302272.FC96_GL001064; -.
DR PATRIC; fig|1302272.5.peg.1070; -.
DR Proteomes; UP000050911; Unassembled WGS sequence.
DR GO; GO:0009346; C:ATP-independent citrate lyase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1080.10; Glutaconate Coenzyme A-transferase; 2.
DR InterPro; IPR006472; Citrate_lyase_asu.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR NCBIfam; TIGR01584; citF; 1.
DR PANTHER; PTHR40596; CITRATE LYASE ALPHA CHAIN; 1.
DR PANTHER; PTHR40596:SF1; CITRATE LYASE ALPHA CHAIN; 1.
DR Pfam; PF04223; CitF; 1.
DR PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 2.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR009451};
KW Lyase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:KRK48745.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000050911};
KW Transferase {ECO:0000256|PIRNR:PIRNR009451}.
SQ SEQUENCE 499 AA; 53957 MW; 8D67479B9E6BD5C0 CRC64;
MNELDYKGFE STEIGNPEIQ RVAPKVHVST GDNKVVSSIE DVVKKTVKDG MTISFHHHFR
NGDFVFNKVM RVIIDAGIQD LTLAPSSLTG VMNDMVIEAI KKGTITNITS SGMRGSLGDF
VSHGGLKNPV IFRSHGNRAR AIEHGDIKID VAFLGVPNAD RLGNANGMNG NAVFGSLGYA
LMDAQYANSV VLLTDNIVPY PNTPASIKQT QVDYVVEVDK VGDPEKIGSG ATRFTKDPKE
LKIAETVNKV ITNSPYFKNG FSFQTGTGGA ALAVTRYLRQ EMIDNKIKAS FALGGITKPT
TDLLEEGLVE KVMDVQDFDK GAADFMHTNP KQQEIDASWY ADPDNKGAMV DQLDVVILSA
LEIDTKFNVN VMSGSDGVIR GAIGGHQDAS TANLTIISAP LIRGRIATVV PDVTTVVTPG
DSVDVLVTEY GIAINPKRDD LKKALSNIPG VPIYDISELQ QMAEKRVGKP TPLQFTDRTV
AMIEYRDGSI IDTIKQVKD
//
