ID A0A0R1QG32_9LACO Unreviewed; 755 AA.
AC A0A0R1QG32;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=FD01_GL001667 {ECO:0000313|EMBL:KRL43521.1};
OS Lacticaseibacillus manihotivorans DSM 13343 = JCM 12514.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=1423769 {ECO:0000313|EMBL:KRL43521.1, ECO:0000313|Proteomes:UP000051790};
RN [1] {ECO:0000313|EMBL:KRL43521.1, ECO:0000313|Proteomes:UP000051790}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 13343 {ECO:0000313|EMBL:KRL43521.1,
RC ECO:0000313|Proteomes:UP000051790};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRL43521.1}.
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DR EMBL; AZEU01000191; KRL43521.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0R1QG32; -.
DR PATRIC; fig|1423769.4.peg.1781; -.
DR Proteomes; UP000051790; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000051790}.
FT DOMAIN 55..314
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 675..751
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 507
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 577
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 755 AA; 84871 MW; 54460DB1E02DB398 CRC64;
MFFTAHNPYT KQCRKRFHET GGFFMADLIA FDAKTQVFHL HNQQISYIFA IEDGGVLGHL
YFGQAVAGYH GERHYPRKDR GFAGNLPVAD PERGFSLDTL PQEISSRGEA DFRIPAFVIC
QADGAQASRF IYASHRIVPG KPKLPGLPAA YVESPDEAAT LIVTVVDQPS RLQADLYYTI
YRDRAVIARS VCFRNPADQP AVTLEQAASV QLDFPAGDYR VLSLPGAHAN ERHLQKEVVG
YGIKQFASTR GTTSHQMNNF IALTAPDADE FHGDVYGIDL VYSGNHALTV EKDQIDQVRV
VAGINPAGFT WELAGGAEFQ TPEALLVYSH QGLNAMSQTY HRLIRERVVR GRYRDQERPI
LVNNWEATYF NFNEAKLRPI VDAAKKLGIE MFVLDDGWFG HRDDDTTSLG DWVVDSHKFP
LGLKHFADYV HNQGLKFGLW VEPEMISKDS DLYRAHPDYL LAVPGRAPSP SRFQYVLDLS
REDVRANIAG QLSDLLDQGY IDYIKWDMNR HLSDIYSHAL PAARQGEVLH RYTLGLYALL
EEMTTKYPDV LWEGCSGGGG RIDTGFAYYM PQSWTSDNTD AISRLGIQYG TSLVYPPNTF
TAHVSVSPNE QTGRSTSLAT RGNVAMSAVF GYELDLTKLT KTEQKQVAAQ VAVYKQIRPL
IQFGHFLRLE SGNDYAWMFV SADQNEALLF TFQLLNDGQP AFHIVKMAGL DPVKQYEDTA
THTVYGGDEL MHMGLYQPLQ RQDFTTHQYH FKAIN
//
